JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling.

Published

Journal Article

Jasmonate and related signalling compounds have a crucial role in both host immunity and development in plants, but the molecular details of the signalling mechanism are poorly understood. Here we identify members of the jasmonate ZIM-domain (JAZ) protein family as key regulators of jasmonate signalling. JAZ1 protein acts to repress transcription of jasmonate-responsive genes. Jasmonate treatment causes JAZ1 degradation and this degradation is dependent on activities of the SCF(COI1) ubiquitin ligase and the 26S proteasome. Furthermore, the jasmonoyl-isoleucine (JA-Ile) conjugate, but not other jasmonate-derivatives such as jasmonate, 12-oxo-phytodienoic acid, or methyl-jasmonate, promotes physical interaction between COI1 and JAZ1 proteins in the absence of other plant proteins. Our results suggest a model in which jasmonate ligands promote the binding of the SCF(COI1) ubiquitin ligase to and subsequent degradation of the JAZ1 repressor protein, and implicate the SCF(COI1)-JAZ1 protein complex as a site of perception of the plant hormone JA-Ile.

Full Text

Duke Authors

Cited Authors

  • Thines, B; Katsir, L; Melotto, M; Niu, Y; Mandaokar, A; Liu, G; Nomura, K; He, SY; Howe, GA; Browse, J

Published Date

  • August 2007

Published In

Volume / Issue

  • 448 / 7154

Start / End Page

  • 661 - 665

PubMed ID

  • 17637677

Pubmed Central ID

  • 17637677

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/nature05960

Language

  • eng