Block of Kir channels by flonicamid disrupts salivary and renal excretion of insect pests.

Journal Article (Journal Article)

Flonicamid is a selective insecticide for the control of sap-sucking insects; it exerts toxic effects by inhibiting insect feeding. However, its molecular target remains elusive. In this study, we functionally characterized NlKir1 channels of the brown planthopper (Nilaparvata lugens) in HEK293 cells. Homomeric NlKir1 channels generated inward-rectifying K+ currents. Flonicamid inhibited NlKir1 channels at nanomolar concentrations. Furthermore, flonicamid inhibited honeydew and salivary secretions of planthoppers, and reduced the renal excretion of female mosquitoes in a dose-dependent manner. The inhibitory effect of flonicamid on fluid secretion of isolated Malpighian tubules from Culex pipiens pullens was comparable to that of the selective Kir1 inhibitor. The observed physiological alterations by flonicamid are likely mediated by Kir1 channels and could lead to the disruption of feeding behaviors and eventually lethality. Our study establishes the Kir1 channel as the target of flonicamid and provided new insights into the mode of action of flonicamid.

Full Text

Duke Authors

Cited Authors

  • Ren, M; Niu, J; Hu, B; Wei, Q; Zheng, C; Tian, X; Gao, C; He, B; Dong, K; Su, J

Published Date

  • August 2018

Published In

Volume / Issue

  • 99 /

Start / End Page

  • 17 - 26

PubMed ID

  • 29842935

Pubmed Central ID

  • 29842935

Electronic International Standard Serial Number (EISSN)

  • 1879-0240

International Standard Serial Number (ISSN)

  • 0965-1748

Digital Object Identifier (DOI)

  • 10.1016/j.ibmb.2018.05.007

Language

  • eng