Effect of a fluvalinate-resistance-associated sodium channel mutation from varroa mites on cockroach sodium channel sensitivity to fluvalinate, a pyrethroid insecticide.
Journal Article (Journal Article)
Fluvalinate is a pyrethroid insecticide that is widely used in the control of the varroa mite (Varroa destructor), an ecto-parasite of the honeybee. Previously we identified four fluvalinate-resistance-associated mutations in the sodium channel gene of the varroa mite. One of the mutations caused a leucine (L) to proline (P) change at 1770 in the linker connecting domains III and IV of the sodium channel. Interestingly, at the position corresponding to the L to P mutation, all known insect (including honeybee) sodium channel proteins already naturally contain a P residue (e.g., P1577 in the cockroach sodium channel BgNa(v)). To determine whether insect sodium channels are less sensitive to fluvalinate than arachnid sodium channels, we replaced P1577 with an L in a BgNa(v) variant (BgNa(v)1-1) and examined the sensitivity of the recombinant channel to fluvalinate. The P1577L substitution did not alter the gating properties of the BgNa(v)1-1 channel expressed in Xenopus oocytes. However, the BgNa(v)1-1(P1577L) channel was five-fold more sensitive to fluvalinate compared with the BgNa(v)1-1 channel. These results not only implicate the L to P mutation in fluvalinate resistance in varroa mites, but also suggest a possible contribution of L1770 to the higher sensitivity of varroa mites to fluvalinate than their insect hosts.
Full Text
Duke Authors
Cited Authors
- Liu, Z; Tan, J; Huang, ZY; Dong, K
Published Date
- November 2006
Published In
Volume / Issue
- 36 / 11
Start / End Page
- 885 - 889
PubMed ID
- 17046602
Electronic International Standard Serial Number (EISSN)
- 1879-0240
International Standard Serial Number (ISSN)
- 0965-1748
Digital Object Identifier (DOI)
- 10.1016/j.ibmb.2006.08.006
Language
- eng