Therapeutic microparticles functionalized with biomimetic cardiac stem cell membranes and secretome.

Journal Article (Journal Article)

Stem cell therapy represents a promising strategy in regenerative medicine. However, cells need to be carefully preserved and processed before usage. In addition, cell transplantation carries immunogenicity and/or tumourigenicity risks. Mounting lines of evidence indicate that stem cells exert their beneficial effects mainly through secretion (of regenerative factors) and membrane-based cell-cell interaction with the injured cells. Here, we fabricate a synthetic cell-mimicking microparticle (CMMP) that recapitulates stem cell functions in tissue repair. CMMPs carry similar secreted proteins and membranes as genuine cardiac stem cells do. In a mouse model of myocardial infarction, injection of CMMPs leads to the preservation of viable myocardium and augmentation of cardiac functions similar to cardiac stem cell therapy. CMMPs (derived from human cells) do not stimulate T-cell infiltration in immuno-competent mice. In conclusion, CMMPs act as 'synthetic stem cells' which mimic the paracrine and biointerfacing activities of natural stem cells in therapeutic cardiac regeneration.

Full Text

Duke Authors

Cited Authors

  • Tang, J; Shen, D; Caranasos, TG; Wang, Z; Vandergriff, AC; Allen, TA; Hensley, MT; Dinh, P-U; Cores, J; Li, T-S; Zhang, J; Kan, Q; Cheng, K

Published Date

  • January 3, 2017

Published In

Volume / Issue

  • 8 /

Start / End Page

  • 13724 -

PubMed ID

  • 28045024

Pubmed Central ID

  • PMC5512648

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/ncomms13724

Language

  • eng