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Obesity alters the collagen organization and mechanical properties of murine cartilage.

Publication ,  Journal Article
Collins, AT; Hu, G; Newman, H; Reinsvold, MH; Goldsmith, MR; Twomey-Kozak, JN; Leddy, HA; Sharma, D; Shen, L; DeFrate, LE; Karner, CM
Published in: Sci Rep
January 15, 2021

Osteoarthritis is a debilitating disease characterized by cartilage degradation and altered cartilage mechanical properties. Furthermore, it is well established that obesity is a primary risk factor for osteoarthritis. The purpose of this study was to investigate the influence of obesity on the mechanical properties of murine knee cartilage. Two-month old wild type mice were fed either a normal diet or a high fat diet for 16 weeks. Atomic force microscopy-based nanoindentation was used to quantify the effective indentation modulus of medial femoral condyle cartilage. Osteoarthritis progression was graded using the OARSI system. Additionally, collagen organization was evaluated with picrosirius red staining imaged using polarized light microscopy. Significant differences between diet groups were assessed using t tests with p < 0.05. Following 16 weeks of a high fat diet, no significant differences in OARSI scoring were detected. However, we detected a significant difference in the effective indentation modulus between diet groups. The reduction in cartilage stiffness is likely the result of disrupted collagen organization in the superficial zone, as indicated by altered birefringence on polarized light microscopy. Collectively, these results suggest obesity is associated with changes in knee cartilage mechanical properties, which may be an early indicator of disease progression.

Duke Scholars

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

January 15, 2021

Volume

11

Issue

1

Start / End Page

1626

Location

England

Related Subject Headings

  • SOX9 Transcription Factor
  • Osteoarthritis
  • Obesity
  • Microscopy, Atomic Force
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Liver
  • Glucose Tolerance Test
  • Elastic Modulus
 

Citation

APA
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MLA
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Collins, A. T., Hu, G., Newman, H., Reinsvold, M. H., Goldsmith, M. R., Twomey-Kozak, J. N., … Karner, C. M. (2021). Obesity alters the collagen organization and mechanical properties of murine cartilage. Sci Rep, 11(1), 1626. https://doi.org/10.1038/s41598-020-80599-1
Collins, Amber T., Guoli Hu, Hunter Newman, Michael H. Reinsvold, Monique R. Goldsmith, John N. Twomey-Kozak, Holly A. Leddy, et al. “Obesity alters the collagen organization and mechanical properties of murine cartilage.Sci Rep 11, no. 1 (January 15, 2021): 1626. https://doi.org/10.1038/s41598-020-80599-1.
Collins AT, Hu G, Newman H, Reinsvold MH, Goldsmith MR, Twomey-Kozak JN, et al. Obesity alters the collagen organization and mechanical properties of murine cartilage. Sci Rep. 2021 Jan 15;11(1):1626.
Collins, Amber T., et al. “Obesity alters the collagen organization and mechanical properties of murine cartilage.Sci Rep, vol. 11, no. 1, Jan. 2021, p. 1626. Pubmed, doi:10.1038/s41598-020-80599-1.
Collins AT, Hu G, Newman H, Reinsvold MH, Goldsmith MR, Twomey-Kozak JN, Leddy HA, Sharma D, Shen L, DeFrate LE, Karner CM. Obesity alters the collagen organization and mechanical properties of murine cartilage. Sci Rep. 2021 Jan 15;11(1):1626.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

January 15, 2021

Volume

11

Issue

1

Start / End Page

1626

Location

England

Related Subject Headings

  • SOX9 Transcription Factor
  • Osteoarthritis
  • Obesity
  • Microscopy, Atomic Force
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Liver
  • Glucose Tolerance Test
  • Elastic Modulus