Sample preparation of the human TRPA1 ion channel for cryo-EM studies.

Book Section

The transient receptor potential ankyrin 1 (TRPA1) ion channel is a member of the TRP channel family that is involved in sensing noxious stimuli that elicit pain and inflammation. Because of its critical physiological role and therapeutic importance, great efforts have been made to understand the structure and mechanism of TRPA1. Several human TRPA1 structures have been reported using single particle cryo-electron microscopy (cryo-EM) over the last 6 years. Here, we present a protocol for the heterologous expression, large-scale purification, and nanodisc reconstitution of the human TRPA1 channel for cryo-EM and biochemical studies.

Full Text

Duke Authors

Cited Authors

  • Suo, Y; Lee, S-Y

Published Date

  • 2021

Volume / Issue

  • 653 /

Start / End Page

  • 75 - 87

PubMed ID

  • 34099182

Digital Object Identifier (DOI)

  • 10.1016/bs.mie.2020.12.018