Single-cell transcriptional profiling of human thymic stroma uncovers novel cellular heterogeneity in the thymic medulla.

Journal Article (Journal Article)

The thymus' key function in the immune system is to provide the necessary environment for the development of diverse and self-tolerant T lymphocytes. While recent evidence suggests that the thymic stroma is comprised of more functionally distinct subpopulations than previously appreciated, the extent of this cellular heterogeneity in the human thymus is not well understood. Here we use single-cell RNA sequencing to comprehensively profile the human thymic stroma across multiple stages of life. Mesenchyme, pericytes and endothelial cells are identified as potential key regulators of thymic epithelial cell differentiation and thymocyte migration. In-depth analyses of epithelial cells reveal the presence of ionocytes as a medullary population, while the expression of tissue-specific antigens is mapped to different subsets of epithelial cells. This work thus provides important insight on how the diversity of thymic cells is established, and how this heterogeneity contributes to the induction of immune tolerance in humans.

Full Text

Duke Authors

Cited Authors

  • Bautista, JL; Cramer, NT; Miller, CN; Chavez, J; Berrios, DI; Byrnes, LE; Germino, J; Ntranos, V; Sneddon, JB; Burt, TD; Gardner, JM; Ye, CJ; Anderson, MS; Parent, AV

Published Date

  • February 17, 2021

Published In

Volume / Issue

  • 12 / 1

Start / End Page

  • 1096 -

PubMed ID

  • 33597545

Pubmed Central ID

  • PMC7889611

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-021-21346-6


  • eng

Conference Location

  • England