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Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis.

Publication ,  Journal Article
Wang, R; Alvarez, DA; Crouch, BT; Pilani, A; Lam, C; Zhu, C; Hughes, P; Katz, D; Haystead, T; Ramanujam, N
Published in: Biomed Opt Express
April 1, 2021

Overexpression of heat shock protein 90 (Hsp90) on the surface of breast cancer cells makes it an attractive molecular biomarker for breast cancer diagnosis. Before a ubiquitous diagnostic method can be established, an understanding of the systematic errors in Hsp90-based imaging is essential. In this study, we investigated three factors that may influence the sensitivity of ex vivo Hsp90 molecular imaging: time-dependent tissue viability, nonspecific diffusion of an Hsp90 specific probe (HS-27), and contact-based imaging. These three factors will be important considerations when designing any diagnostic imaging strategy based on fluorescence imaging of a molecular target on tissue samples.

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Published In

Biomed Opt Express

DOI

ISSN

2156-7085

Publication Date

April 1, 2021

Volume

12

Issue

4

Start / End Page

2299 / 2311

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics
 

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Wang, R., Alvarez, D. A., Crouch, B. T., Pilani, A., Lam, C., Zhu, C., … Ramanujam, N. (2021). Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis. Biomed Opt Express, 12(4), 2299–2311. https://doi.org/10.1364/BOE.418818
Wang, Roujia, Daniel A. Alvarez, Brian T. Crouch, Aditi Pilani, Christopher Lam, Caigang Zhu, Philip Hughes, David Katz, Timothy Haystead, and Nirmala Ramanujam. “Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis.Biomed Opt Express 12, no. 4 (April 1, 2021): 2299–2311. https://doi.org/10.1364/BOE.418818.
Wang R, Alvarez DA, Crouch BT, Pilani A, Lam C, Zhu C, et al. Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis. Biomed Opt Express. 2021 Apr 1;12(4):2299–311.
Wang, Roujia, et al. “Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis.Biomed Opt Express, vol. 12, no. 4, Apr. 2021, pp. 2299–311. Pubmed, doi:10.1364/BOE.418818.
Wang R, Alvarez DA, Crouch BT, Pilani A, Lam C, Zhu C, Hughes P, Katz D, Haystead T, Ramanujam N. Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis. Biomed Opt Express. 2021 Apr 1;12(4):2299–2311.
Journal cover image

Published In

Biomed Opt Express

DOI

ISSN

2156-7085

Publication Date

April 1, 2021

Volume

12

Issue

4

Start / End Page

2299 / 2311

Location

United States

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics