Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis.

Journal Article (Journal Article)

Overexpression of heat shock protein 90 (Hsp90) on the surface of breast cancer cells makes it an attractive molecular biomarker for breast cancer diagnosis. Before a ubiquitous diagnostic method can be established, an understanding of the systematic errors in Hsp90-based imaging is essential. In this study, we investigated three factors that may influence the sensitivity of ex vivo Hsp90 molecular imaging: time-dependent tissue viability, nonspecific diffusion of an Hsp90 specific probe (HS-27), and contact-based imaging. These three factors will be important considerations when designing any diagnostic imaging strategy based on fluorescence imaging of a molecular target on tissue samples.

Full Text

Duke Authors

Cited Authors

  • Wang, R; Alvarez, DA; Crouch, BT; Pilani, A; Lam, C; Zhu, C; Hughes, P; Katz, D; Haystead, T; Ramanujam, N

Published Date

  • April 1, 2021

Published In

Volume / Issue

  • 12 / 4

Start / End Page

  • 2299 - 2311

PubMed ID

  • 33996230

Pubmed Central ID

  • PMC8086448

International Standard Serial Number (ISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/BOE.418818


  • eng

Conference Location

  • United States