Calcium signals and calpain-dependent necrosis are essential for release of coxsackievirus B from polarized intestinal epithelial cells.

Journal Article (Journal Article)

Coxsackievirus B (CVB), a member of the enterovirus family, targets the polarized epithelial cells lining the intestinal tract early in infection. Although the polarized epithelium functions as a protective barrier, this barrier is likely exploited by CVB to promote viral entry and subsequent egress. Here we show that, in contrast to nonpolarized cells, CVB-infected polarized intestinal Caco-2 cells undergo nonapoptotic necrotic cell death triggered by inositol 1,4,5-trisphosphate receptor-dependent calcium release. We further show that CVB-induced cellular necrosis depends on the Ca(2+)-activated protease calpain-2 and that this protease is involved in CVB-induced disruption of the junctional complex and rearrangements of the actin cytoskeleton. Our study illustrates the cell signaling pathways hijacked by CVB, and perhaps other viral pathogens, to promote their replication and spread in polarized cell types.

Full Text

Duke Authors

Cited Authors

  • Bozym, RA; Patel, K; White, C; Cheung, K-H; Bergelson, JM; Morosky, SA; Coyne, CB

Published Date

  • September 2011

Published In

Volume / Issue

  • 22 / 17

Start / End Page

  • 3010 - 3021

PubMed ID

  • 21737691

Pubmed Central ID

  • PMC3164450

Electronic International Standard Serial Number (EISSN)

  • 1939-4586

Digital Object Identifier (DOI)

  • 10.1091/mbc.E11-02-0094

Language

  • eng

Conference Location

  • United States