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Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells.

Publication ,  Journal Article
Bozym, RA; Morosky, SA; Kim, KS; Cherry, S; Coyne, CB
Published in: PLoS Pathog
October 7, 2010

Group B coxsackieviruses (CVB) are associated with viral-induced heart disease and are among the leading causes of aseptic meningitis worldwide. Here we show that CVB entry into polarized brain microvasculature and aortic endothelial cells triggers a depletion of intracellular calcium stores initiated through viral attachment to the apical attachment factor decay-accelerating factor. Calcium release was dependent upon a signaling cascade that required the activity of the Src family of tyrosine kinases, phospholipase C, and the inositol 1,4,5-trisphosphate receptor isoform 3. CVB-mediated calcium release was required for the activation of calpain-2, a calcium-dependent cysteine protease, which controlled the vesicular trafficking of internalized CVB particles. These data point to a specific role for calcium signaling in CVB entry into polarized endothelial monolayers and highlight the unique signaling mechanisms used by these viruses to cross endothelial barriers.

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Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

October 7, 2010

Volume

6

Issue

10

Start / End Page

e1001135

Location

United States

Related Subject Headings

  • Virus Internalization
  • Virology
  • Phospholipase C gamma
  • Intracellular Space
  • Inositol 1,4,5-Trisphosphate Receptors
  • Humans
  • Epithelium
  • Enterovirus
  • Endothelium, Vascular
  • Endothelial Cells
 

Citation

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Bozym, R. A., Morosky, S. A., Kim, K. S., Cherry, S., & Coyne, C. B. (2010). Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells. PLoS Pathog, 6(10), e1001135. https://doi.org/10.1371/journal.ppat.1001135
Bozym, Rebecca A., Stefanie A. Morosky, Kwang S. Kim, Sara Cherry, and Carolyn B. Coyne. “Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells.PLoS Pathog 6, no. 10 (October 7, 2010): e1001135. https://doi.org/10.1371/journal.ppat.1001135.
Bozym RA, Morosky SA, Kim KS, Cherry S, Coyne CB. Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells. PLoS Pathog. 2010 Oct 7;6(10):e1001135.
Bozym, Rebecca A., et al. “Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells.PLoS Pathog, vol. 6, no. 10, Oct. 2010, p. e1001135. Pubmed, doi:10.1371/journal.ppat.1001135.
Bozym RA, Morosky SA, Kim KS, Cherry S, Coyne CB. Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells. PLoS Pathog. 2010 Oct 7;6(10):e1001135.

Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

October 7, 2010

Volume

6

Issue

10

Start / End Page

e1001135

Location

United States

Related Subject Headings

  • Virus Internalization
  • Virology
  • Phospholipase C gamma
  • Intracellular Space
  • Inositol 1,4,5-Trisphosphate Receptors
  • Humans
  • Epithelium
  • Enterovirus
  • Endothelium, Vascular
  • Endothelial Cells