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Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers.

Publication ,  Journal Article
Coyne, CB; Bozym, R; Morosky, SA; Hanna, SL; Mukherjee, A; Tudor, M; Kim, KS; Cherry, S
Published in: Cell Host Microbe
January 20, 2011

Enteroviruses, including coxsackievirus B (CVB) and poliovirus (PV), can access the CNS through the blood brain barrier (BBB) endothelium to cause aseptic meningitis. To identify cellular components required for CVB and PV infection of human brain microvascular endothelial cells, an in vitro BBB model, we performed comparative RNAi screens and identified 117 genes that influenced infection. Whereas a large proportion of genes whose depletion enhanced infection (17 of 22) were broadly antienteroviral, only 46 of the 95 genes whose depletion inhibited infection were required by both CVB and PV and included components of cell signaling pathways such as adenylate cyclases. Downregulation of genes including Rab GTPases, Src tyrosine kinases, and tyrosine phosphatases displayed specificity in their requirement for either CVB or PV infection. These findings highlight the pathways hijacked by enteroviruses for entry and replication in the BBB endothelium, a specialized and clinically relevant cell type for these viruses.

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Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

January 20, 2011

Volume

9

Issue

1

Start / End Page

70 / 82

Location

United States

Related Subject Headings

  • Poliovirus
  • Models, Biological
  • Mass Screening
  • Immunology
  • Humans
  • Host-Pathogen Interactions
  • Gene Silencing
  • Enterovirus B, Human
  • Endothelial Cells
  • Blood-Brain Barrier
 

Citation

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Coyne, C. B., Bozym, R., Morosky, S. A., Hanna, S. L., Mukherjee, A., Tudor, M., … Cherry, S. (2011). Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers. Cell Host Microbe, 9(1), 70–82. https://doi.org/10.1016/j.chom.2011.01.001
Coyne, Carolyn B., Rebecca Bozym, Stefanie A. Morosky, Sheri L. Hanna, Amitava Mukherjee, Matthew Tudor, Kwang Sik Kim, and Sara Cherry. “Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers.Cell Host Microbe 9, no. 1 (January 20, 2011): 70–82. https://doi.org/10.1016/j.chom.2011.01.001.
Coyne CB, Bozym R, Morosky SA, Hanna SL, Mukherjee A, Tudor M, et al. Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers. Cell Host Microbe. 2011 Jan 20;9(1):70–82.
Coyne, Carolyn B., et al. “Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers.Cell Host Microbe, vol. 9, no. 1, Jan. 2011, pp. 70–82. Pubmed, doi:10.1016/j.chom.2011.01.001.
Coyne CB, Bozym R, Morosky SA, Hanna SL, Mukherjee A, Tudor M, Kim KS, Cherry S. Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers. Cell Host Microbe. 2011 Jan 20;9(1):70–82.
Journal cover image

Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

January 20, 2011

Volume

9

Issue

1

Start / End Page

70 / 82

Location

United States

Related Subject Headings

  • Poliovirus
  • Models, Biological
  • Mass Screening
  • Immunology
  • Humans
  • Host-Pathogen Interactions
  • Gene Silencing
  • Enterovirus B, Human
  • Endothelial Cells
  • Blood-Brain Barrier