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A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.

Publication ,  Journal Article
Moser, TS; Jones, RG; Thompson, CB; Coyne, CB; Cherry, S
Published in: PLoS Pathog
June 17, 2010

Poxviruses include medically important human pathogens, yet little is known about the specific cellular factors essential for their replication. To identify genes essential for poxvirus infection, we used high-throughput RNA interference to screen the Drosophila kinome for factors required for vaccinia infection. We identified seven genes including the three subunits of AMPK as promoting vaccinia infection. AMPK not only facilitated infection in insect cells, but also in mammalian cells. Moreover, we found that AMPK is required for macropinocytosis, a major endocytic entry pathway for vaccinia. Furthermore, we show that AMPK contributes to other virus-independent actin-dependent processes including lamellipodia formation and wound healing, independent of the known AMPK activators LKB1 and CaMKK. Therefore, AMPK plays a highly conserved role in poxvirus infection and actin dynamics independent of its role as an energy regulator.

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Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

June 17, 2010

Volume

6

Issue

6

Start / End Page

e1000954

Location

United States

Related Subject Headings

  • Wound Healing
  • Virus Replication
  • Virus Internalization
  • Virology
  • Vaccinia virus
  • Vaccinia
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • RNA Interference
  • Pseudopodia
 

Citation

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Moser, T. S., Jones, R. G., Thompson, C. B., Coyne, C. B., & Cherry, S. (2010). A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. PLoS Pathog, 6(6), e1000954. https://doi.org/10.1371/journal.ppat.1000954
Moser, Theresa S., Russell G. Jones, Craig B. Thompson, Carolyn B. Coyne, and Sara Cherry. “A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.PLoS Pathog 6, no. 6 (June 17, 2010): e1000954. https://doi.org/10.1371/journal.ppat.1000954.
Moser TS, Jones RG, Thompson CB, Coyne CB, Cherry S. A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. PLoS Pathog. 2010 Jun 17;6(6):e1000954.
Moser, Theresa S., et al. “A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.PLoS Pathog, vol. 6, no. 6, June 2010, p. e1000954. Pubmed, doi:10.1371/journal.ppat.1000954.
Moser TS, Jones RG, Thompson CB, Coyne CB, Cherry S. A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics. PLoS Pathog. 2010 Jun 17;6(6):e1000954.

Published In

PLoS Pathog

DOI

EISSN

1553-7374

Publication Date

June 17, 2010

Volume

6

Issue

6

Start / End Page

e1000954

Location

United States

Related Subject Headings

  • Wound Healing
  • Virus Replication
  • Virus Internalization
  • Virology
  • Vaccinia virus
  • Vaccinia
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • RNA Interference
  • Pseudopodia