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SARS-CoV-2 vaccines elicit durable immune responses in infant rhesus macaques.

Publication ,  Journal Article
Garrido, C; Curtis, AD; Dennis, M; Pathak, SH; Gao, H; Montefiori, D; Tomai, M; Fox, CB; Kozlowski, PA; Scobey, T; Munt, JE; Mallory, ML ...
Published in: Sci Immunol
June 15, 2021

The inclusion of infants in the SARS-CoV-2 vaccine roll-out is important to prevent severe complications of pediatric SARS-CoV-2 infections and to limit transmission and could possibly be implemented via the global pediatric vaccine schedule. However, age-dependent differences in immune function require careful evaluation of novel vaccines in the pediatric population. Toward this goal, we assessed the safety and immunogenicity of two SARS-CoV-2 vaccines. Two groups of 8 infant rhesus macaques (RMs) were immunized intramuscularly at weeks 0 and 4 with stabilized prefusion SARS-CoV-2 S-2P spike (S) protein encoded by mRNA encapsulated in lipid nanoparticles (mRNA-LNP) or the purified S protein mixed with 3M-052, a synthetic TLR7/8 agonist in a squalene emulsion (Protein+3M-052-SE). Neither vaccine induced adverse effects. Both vaccines elicited high magnitude IgG binding to RBD, N terminus domain, S1, and S2, ACE2 blocking activity, and high neutralizing antibody titers, all peaking at week 6. S-specific memory B cells were detected by week 4 and S-specific T cell responses were dominated by the production of IL-17, IFN-γ, or TNF-α. Antibody and cellular responses were stable through week 22. The immune responses for the mRNA-LNP vaccine were of a similar magnitude to those elicited by the Moderna mRNA-1273 vaccine in adults. The S-2P mRNA-LNP and Protein-3M-052-SE vaccines were well-tolerated and highly immunogenic in infant RMs, providing proof-of concept for a pediatric SARS-CoV-2 vaccine with the potential for durable immunity that might decrease the transmission of SARS-CoV-2 and mitigate the ongoing health and socioeconomic impacts of COVID-19.

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Published In

Sci Immunol

DOI

EISSN

2470-9468

Publication Date

June 15, 2021

Volume

6

Issue

60

Location

United States

Related Subject Headings

  • Spike Glycoprotein, Coronavirus
  • SARS-CoV-2
  • Macaca mulatta
  • COVID-19 Vaccines
  • COVID-19
  • Antibodies, Viral
  • Antibodies, Neutralizing
  • Animals, Newborn
  • Animals
  • 3204 Immunology
 

Citation

APA
Chicago
ICMJE
MLA
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Garrido, C., Curtis, A. D., Dennis, M., Pathak, S. H., Gao, H., Montefiori, D., … Permar, S. R. (2021). SARS-CoV-2 vaccines elicit durable immune responses in infant rhesus macaques. Sci Immunol, 6(60). https://doi.org/10.1126/sciimmunol.abj3684
Garrido, Carolina, Alan D. Curtis, Maria Dennis, Sachi H. Pathak, Hongmei Gao, David Montefiori, Mark Tomai, et al. “SARS-CoV-2 vaccines elicit durable immune responses in infant rhesus macaques.Sci Immunol 6, no. 60 (June 15, 2021). https://doi.org/10.1126/sciimmunol.abj3684.
Garrido C, Curtis AD, Dennis M, Pathak SH, Gao H, Montefiori D, et al. SARS-CoV-2 vaccines elicit durable immune responses in infant rhesus macaques. Sci Immunol. 2021 Jun 15;6(60).
Garrido, Carolina, et al. “SARS-CoV-2 vaccines elicit durable immune responses in infant rhesus macaques.Sci Immunol, vol. 6, no. 60, June 2021. Pubmed, doi:10.1126/sciimmunol.abj3684.
Garrido C, Curtis AD, Dennis M, Pathak SH, Gao H, Montefiori D, Tomai M, Fox CB, Kozlowski PA, Scobey T, Munt JE, Mallory ML, Saha PT, Hudgens MG, Lindesmith LC, Baric RS, Abiona OM, Graham B, Corbett KS, Edwards D, Carfi A, Fouda G, Van Rompay KKA, De Paris K, Permar SR. SARS-CoV-2 vaccines elicit durable immune responses in infant rhesus macaques. Sci Immunol. 2021 Jun 15;6(60).

Published In

Sci Immunol

DOI

EISSN

2470-9468

Publication Date

June 15, 2021

Volume

6

Issue

60

Location

United States

Related Subject Headings

  • Spike Glycoprotein, Coronavirus
  • SARS-CoV-2
  • Macaca mulatta
  • COVID-19 Vaccines
  • COVID-19
  • Antibodies, Viral
  • Antibodies, Neutralizing
  • Animals, Newborn
  • Animals
  • 3204 Immunology