Broad immunophenotyping of the murine brain tumor microenvironment.
Publication
, Journal Article
Tomaszewski, WH; Waibl-Polania, J; Miggelbrink, AM; Chakraborty, MA; Fecci, PE; Sampson, JH; Gunn, MD
Published in: J Immunol Methods
December 2021
Here we present a 14-color flow cytometry panel for the evaluation of 13 myeloid and lymphoid populations within murine glioblastoma samples. Reagents, processing protocols, and downstream analyses were thoroughly validated and optimized to resolve the following populations: T cells (CD4, CD8, CD3), B cells (B220), NK cells (NK1.1), neutrophils (Ly6G), classical and non-classical monocytes (Ly6c, CD43), macrophages (F4/80, CD11b), microglia (CD45-lo, CD11b), and dendritic cells (DCs) (CD11c, MHC class II). In addition, this panel leaves Alexa Fluor 488/FITC open for the inclusion of fluorescent reporters or congenic marker staining.
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Published In
J Immunol Methods
DOI
EISSN
1872-7905
Publication Date
December 2021
Volume
499
Start / End Page
113158
Location
Netherlands
Related Subject Headings
- Tumor Microenvironment
- Mice, Inbred C57BL
- Mice
- Immunophenotyping
- Immunology
- Brain Neoplasms
- Animals
- 3204 Immunology
- 3101 Biochemistry and cell biology
- 1113 Opthalmology and Optometry
Citation
APA
Chicago
ICMJE
MLA
NLM
Tomaszewski, W. H., Waibl-Polania, J., Miggelbrink, A. M., Chakraborty, M. A., Fecci, P. E., Sampson, J. H., & Gunn, M. D. (2021). Broad immunophenotyping of the murine brain tumor microenvironment. J Immunol Methods, 499, 113158. https://doi.org/10.1016/j.jim.2021.113158
Tomaszewski, W. H., J. Waibl-Polania, A. M. Miggelbrink, M. A. Chakraborty, P. E. Fecci, J. H. Sampson, and M. D. Gunn. “Broad immunophenotyping of the murine brain tumor microenvironment.” J Immunol Methods 499 (December 2021): 113158. https://doi.org/10.1016/j.jim.2021.113158.
Tomaszewski WH, Waibl-Polania J, Miggelbrink AM, Chakraborty MA, Fecci PE, Sampson JH, et al. Broad immunophenotyping of the murine brain tumor microenvironment. J Immunol Methods. 2021 Dec;499:113158.
Tomaszewski, W. H., et al. “Broad immunophenotyping of the murine brain tumor microenvironment.” J Immunol Methods, vol. 499, Dec. 2021, p. 113158. Pubmed, doi:10.1016/j.jim.2021.113158.
Tomaszewski WH, Waibl-Polania J, Miggelbrink AM, Chakraborty MA, Fecci PE, Sampson JH, Gunn MD. Broad immunophenotyping of the murine brain tumor microenvironment. J Immunol Methods. 2021 Dec;499:113158.
Published In
J Immunol Methods
DOI
EISSN
1872-7905
Publication Date
December 2021
Volume
499
Start / End Page
113158
Location
Netherlands
Related Subject Headings
- Tumor Microenvironment
- Mice, Inbred C57BL
- Mice
- Immunophenotyping
- Immunology
- Brain Neoplasms
- Animals
- 3204 Immunology
- 3101 Biochemistry and cell biology
- 1113 Opthalmology and Optometry