Broad immunophenotyping of the murine brain tumor microenvironment.

Journal Article (Journal Article)

Here we present a 14-color flow cytometry panel for the evaluation of 13 myeloid and lymphoid populations within murine glioblastoma samples. Reagents, processing protocols, and downstream analyses were thoroughly validated and optimized to resolve the following populations: T cells (CD4, CD8, CD3), B cells (B220), NK cells (NK1.1), neutrophils (Ly6G), classical and non-classical monocytes (Ly6c, CD43), macrophages (F4/80, CD11b), microglia (CD45-lo, CD11b), and dendritic cells (DCs) (CD11c, MHC class II). In addition, this panel leaves Alexa Fluor 488/FITC open for the inclusion of fluorescent reporters or congenic marker staining.

Full Text

Duke Authors

Cited Authors

  • Tomaszewski, WH; Waibl-Polania, J; Miggelbrink, AM; Chakraborty, MA; Fecci, PE; Sampson, JH; Gunn, MD

Published Date

  • December 2021

Published In

Volume / Issue

  • 499 /

Start / End Page

  • 113158 -

PubMed ID

  • 34597618

Pubmed Central ID

  • PMC8608727

Electronic International Standard Serial Number (EISSN)

  • 1872-7905

Digital Object Identifier (DOI)

  • 10.1016/j.jim.2021.113158

Language

  • eng

Conference Location

  • Netherlands