Broad immunophenotyping of the murine brain tumor microenvironment.
Journal Article (Journal Article)
Here we present a 14-color flow cytometry panel for the evaluation of 13 myeloid and lymphoid populations within murine glioblastoma samples. Reagents, processing protocols, and downstream analyses were thoroughly validated and optimized to resolve the following populations: T cells (CD4, CD8, CD3), B cells (B220), NK cells (NK1.1), neutrophils (Ly6G), classical and non-classical monocytes (Ly6c, CD43), macrophages (F4/80, CD11b), microglia (CD45-lo, CD11b), and dendritic cells (DCs) (CD11c, MHC class II). In addition, this panel leaves Alexa Fluor 488/FITC open for the inclusion of fluorescent reporters or congenic marker staining.
Full Text
Duke Authors
Cited Authors
- Tomaszewski, WH; Waibl-Polania, J; Miggelbrink, AM; Chakraborty, MA; Fecci, PE; Sampson, JH; Gunn, MD
Published Date
- December 2021
Published In
Volume / Issue
- 499 /
Start / End Page
- 113158 -
PubMed ID
- 34597618
Pubmed Central ID
- PMC8608727
Electronic International Standard Serial Number (EISSN)
- 1872-7905
Digital Object Identifier (DOI)
- 10.1016/j.jim.2021.113158
Language
- eng
Conference Location
- Netherlands