Differential dynamics and activity-dependent regulation of alpha- and beta-neurexins at developing GABAergic synapses.

Journal Article (Journal Article)

Neurexins (NRXs) and neuroligins are key synaptic adhesion molecules that also recruit synaptic signaling machineries. Neurexins consist of α- and β-isoforms, but how they couple synaptic transmission and adhesion to regulate activity-dependent synapse development remains unclear, in part because of poor understanding of their cell biology and regulation in the relevant neurons. Here, we examined the subaxonal localization, dynamics, and regulation of NRX1α and NRX1β in cortical perisomatic inhibitory synapses. Both isoforms are delivered to presynaptic terminals but show significant and different turnover rate at the membrane. Although NRX1α is highly diffuse along developing axons and filopodia, NRX1β is strictly anchored at terminals through binding to postsynaptic ligands. The turnover rate of NRX1β is attenuated by neural activity and presynaptic GABA(B) receptors. NRXs, thus, are intrinsically dynamic but are stabilized by local transmitter release. Such an activity-adjusted adhesion system seems ideally suited to rapidly explore and validate synaptic partners guided by synaptic transmission.

Full Text

Duke Authors

Cited Authors

  • Fu, Y; Huang, ZJ

Published Date

  • December 28, 2010

Published In

Volume / Issue

  • 107 / 52

Start / End Page

  • 22699 - 22704

PubMed ID

  • 21149722

Pubmed Central ID

  • PMC3012487

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.1011233108

Language

  • eng

Conference Location

  • United States