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Cortical representations of olfactory input by trans-synaptic tracing.

Publication ,  Journal Article
Miyamichi, K; Amat, F; Moussavi, F; Wang, C; Wickersham, I; Wall, NR; Taniguchi, H; Tasic, B; Huang, ZJ; He, Z; Callaway, EM; Horowitz, MA; Luo, L
Published in: Nature
April 14, 2011

In the mouse, each class of olfactory receptor neurons expressing a given odorant receptor has convergent axonal projections to two specific glomeruli in the olfactory bulb, thereby creating an odour map. However, it is unclear how this map is represented in the olfactory cortex. Here we combine rabies-virus-dependent retrograde mono-trans-synaptic labelling with genetics to control the location, number and type of 'starter' cortical neurons, from which we trace their presynaptic neurons. We find that individual cortical neurons receive input from multiple mitral cells representing broadly distributed glomeruli. Different cortical areas represent the olfactory bulb input differently. For example, the cortical amygdala preferentially receives dorsal olfactory bulb input, whereas the piriform cortex samples the whole olfactory bulb without obvious bias. These differences probably reflect different functions of these cortical areas in mediating innate odour preference or associative memory. The trans-synaptic labelling method described here should be widely applicable to mapping connections throughout the mouse nervous system.

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Published In

Nature

DOI

EISSN

1476-4687

Publication Date

April 14, 2011

Volume

472

Issue

7342

Start / End Page

191 / 196

Location

England

Related Subject Headings

  • Synapses
  • Rabies virus
  • Olfactory Receptor Neurons
  • Olfactory Perception
  • Olfactory Pathways
  • Olfactory Bulb
  • Odorants
  • Neuroanatomical Tract-Tracing Techniques
  • Mice, Transgenic
  • Mice
 

Citation

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Miyamichi, K., Amat, F., Moussavi, F., Wang, C., Wickersham, I., Wall, N. R., … Luo, L. (2011). Cortical representations of olfactory input by trans-synaptic tracing. Nature, 472(7342), 191–196. https://doi.org/10.1038/nature09714
Miyamichi, Kazunari, Fernando Amat, Farshid Moussavi, Chen Wang, Ian Wickersham, Nicholas R. Wall, Hiroki Taniguchi, et al. “Cortical representations of olfactory input by trans-synaptic tracing.Nature 472, no. 7342 (April 14, 2011): 191–96. https://doi.org/10.1038/nature09714.
Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, et al. Cortical representations of olfactory input by trans-synaptic tracing. Nature. 2011 Apr 14;472(7342):191–6.
Miyamichi, Kazunari, et al. “Cortical representations of olfactory input by trans-synaptic tracing.Nature, vol. 472, no. 7342, Apr. 2011, pp. 191–96. Pubmed, doi:10.1038/nature09714.
Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Cortical representations of olfactory input by trans-synaptic tracing. Nature. 2011 Apr 14;472(7342):191–196.
Journal cover image

Published In

Nature

DOI

EISSN

1476-4687

Publication Date

April 14, 2011

Volume

472

Issue

7342

Start / End Page

191 / 196

Location

England

Related Subject Headings

  • Synapses
  • Rabies virus
  • Olfactory Receptor Neurons
  • Olfactory Perception
  • Olfactory Pathways
  • Olfactory Bulb
  • Odorants
  • Neuroanatomical Tract-Tracing Techniques
  • Mice, Transgenic
  • Mice