A resource of Cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex.

Journal Article (Journal Article)

A key obstacle to understanding neural circuits in the cerebral cortex is that of unraveling the diversity of GABAergic interneurons. This diversity poses general questions for neural circuit analysis: how are these interneuron cell types generated and assembled into stereotyped local circuits and how do they differentially contribute to circuit operations that underlie cortical functions ranging from perception to cognition? Using genetic engineering in mice, we have generated and characterized approximately 20 Cre and inducible CreER knockin driver lines that reliably target major classes and lineages of GABAergic neurons. More select populations are captured by intersection of Cre and Flp drivers. Genetic targeting allows reliable identification, monitoring, and manipulation of cortical GABAergic neurons, thereby enabling a systematic and comprehensive analysis from cell fate specification, migration, and connectivity, to their functions in network dynamics and behavior. As such, this approach will accelerate the study of GABAergic circuits throughout the mammalian brain.

Full Text

Duke Authors

Cited Authors

  • Taniguchi, H; He, M; Wu, P; Kim, S; Paik, R; Sugino, K; Kvitsiani, D; Fu, Y; Lu, J; Lin, Y; Miyoshi, G; Shima, Y; Fishell, G; Nelson, SB; Huang, ZJ

Published Date

  • September 22, 2011

Published In

Volume / Issue

  • 71 / 6

Start / End Page

  • 995 - 1013

PubMed ID

  • 21943598

Pubmed Central ID

  • PMC3779648

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2011.07.026

Language

  • eng

Conference Location

  • United States