PER protein interactions and temperature compensation of a circadian clock in Drosophila.

Journal Article (Journal Article)

The periods of circadian clocks are relatively temperature-insensitive. Indeed, the perL mutation in the Drosophila melanogaster period gene, a central component of the clock, affects temperature compensation as well as period length. The per protein (PER) contains a dimerization domain (PAS) within which the perL mutation is located. Amino acid substitutions at the perL position rendered PER dimerization temperature-sensitive. In addition, another region of PER interacted with PAS, and the perL mutation enhanced this putative intramolecular interaction, which may compete with PAS-PAS intermolecular interactions. Therefore, temperature compensation of circadian period in Drosophila may be due in part to temperature-independent PER activity, which is based on competition between inter- and intramolecular interactions with similar temperature coefficients.

Full Text

Duke Authors

Cited Authors

  • Huang, ZJ; Curtin, KD; Rosbash, M

Published Date

  • February 24, 1995

Published In

Volume / Issue

  • 267 / 5201

Start / End Page

  • 1169 - 1172

PubMed ID

  • 7855598

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.7855598

Language

  • eng

Conference Location

  • United States