Detection and Quantification of Enteric Pathogens in Aerosols Near Open Wastewater Canals in Cities with Poor Sanitation.

Journal Article (Journal Article)

Urban sanitation infrastructure is inadequate in many low-income countries, leading to the presence of highly concentrated, uncontained fecal waste streams in densely populated areas. Combined with mechanisms of aerosolization, airborne transport of enteric microbes and their genetic material is possible in such settings but remains poorly characterized. We detected and quantified enteric pathogen-associated gene targets in aerosol samples near open wastewater canals (OWCs) or impacted (receiving sewage or wastewater) surface waters and control sites in La Paz, Bolivia; Kanpur, India; and Atlanta, USA, via multiplex reverse-transcription qPCR (37 targets) and ddPCR (13 targets). We detected a wide range of enteric targets, some not previously reported in extramural urban aerosols, with more frequent detections of all enteric targets at higher densities in La Paz and Kanpur near OWCs. We report density estimates ranging up to 4.7 × 102 gc per mair3 across all targets including heat-stable enterotoxigenic Escherichia coli, Campylobacter jejuni, enteroinvasive E. coli/Shigella spp., Salmonella spp., norovirus, and Cryptosporidium spp. Estimated 25, 76, and 0% of samples containing positive pathogen detects were accompanied by culturable E. coli in La Paz, Kanpur, and Atlanta, respectively, suggesting potential for viability of enteric microbes at the point of sampling. Airborne transmission of enteric pathogens merits further investigation in cities with poor sanitation.

Full Text

Duke Authors

Cited Authors

  • Ginn, O; Rocha-Melogno, L; Bivins, A; Lowry, S; Cardelino, M; Nichols, D; Tripathi, SN; Soria, F; Andrade, M; Bergin, M; Deshusses, MA; Brown, J

Published Date

  • November 2021

Published In

Volume / Issue

  • 55 / 21

Start / End Page

  • 14758 - 14771

PubMed ID

  • 34669386

Electronic International Standard Serial Number (EISSN)

  • 1520-5851

International Standard Serial Number (ISSN)

  • 0013-936X

Digital Object Identifier (DOI)

  • 10.1021/acs.est.1c05060

Language

  • eng