Zebrafish developmental screening of the ToxCast™ Phase I chemical library.

Journal Article (Journal Article)

Zebrafish (Danio rerio) is an emerging toxicity screening model for both human health and ecology. As part of the Computational Toxicology Research Program of the U.S. EPA, the toxicity of the 309 ToxCast™ Phase I chemicals was assessed using a zebrafish screen for developmental toxicity. All exposures were by immersion from 6-8 h post fertilization (hpf) to 5 days post fertilization (dpf); nominal concentration range of 1 nM-80 μM. On 6 dpf larvae were assessed for death and overt structural defects. Results revealed that the majority (62%) of chemicals were toxic to the developing zebrafish; both toxicity incidence and potency was correlated with chemical class and hydrophobicity (logP); and inter-and intra-plate replicates showed good agreement. The zebrafish embryo screen, by providing an integrated model of the developing vertebrate, compliments the ToxCast assay portfolio and has the potential to provide information relative to overt and organismal toxicity.

Full Text

Duke Authors

Cited Authors

  • Padilla, S; Corum, D; Padnos, B; Hunter, DL; Beam, A; Houck, KA; Sipes, N; Kleinstreuer, N; Knudsen, T; Dix, DJ; Reif, DM

Published Date

  • April 2012

Published In

Volume / Issue

  • 33 / 2

Start / End Page

  • 174 - 187

PubMed ID

  • 22182468

Electronic International Standard Serial Number (EISSN)

  • 1873-1708

International Standard Serial Number (ISSN)

  • 0890-6238

Digital Object Identifier (DOI)

  • 10.1016/j.reprotox.2011.10.018

Language

  • eng