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Zebrafish developmental screening of the ToxCast™ Phase I chemical library.

Publication ,  Journal Article
Padilla, S; Corum, D; Padnos, B; Hunter, DL; Beam, A; Houck, KA; Sipes, N; Kleinstreuer, N; Knudsen, T; Dix, DJ; Reif, DM
Published in: Reproductive toxicology (Elmsford, N.Y.)
April 2012

Zebrafish (Danio rerio) is an emerging toxicity screening model for both human health and ecology. As part of the Computational Toxicology Research Program of the U.S. EPA, the toxicity of the 309 ToxCast™ Phase I chemicals was assessed using a zebrafish screen for developmental toxicity. All exposures were by immersion from 6-8 h post fertilization (hpf) to 5 days post fertilization (dpf); nominal concentration range of 1 nM-80 μM. On 6 dpf larvae were assessed for death and overt structural defects. Results revealed that the majority (62%) of chemicals were toxic to the developing zebrafish; both toxicity incidence and potency was correlated with chemical class and hydrophobicity (logP); and inter-and intra-plate replicates showed good agreement. The zebrafish embryo screen, by providing an integrated model of the developing vertebrate, compliments the ToxCast assay portfolio and has the potential to provide information relative to overt and organismal toxicity.

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Published In

Reproductive toxicology (Elmsford, N.Y.)

DOI

EISSN

1873-1708

ISSN

0890-6238

Publication Date

April 2012

Volume

33

Issue

2

Start / End Page

174 / 187

Related Subject Headings

  • Zebrafish
  • Toxicology
  • Toxicity Tests
  • Teratogens
  • Small Molecule Libraries
  • Pesticides
  • Models, Animal
  • Environmental Pollutants
  • Embryo, Nonmammalian
  • Animals
 

Citation

APA
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Padilla, S., Corum, D., Padnos, B., Hunter, D. L., Beam, A., Houck, K. A., … Reif, D. M. (2012). Zebrafish developmental screening of the ToxCast™ Phase I chemical library. Reproductive Toxicology (Elmsford, N.Y.), 33(2), 174–187. https://doi.org/10.1016/j.reprotox.2011.10.018
Padilla, S., D. Corum, B. Padnos, D. L. Hunter, A. Beam, K. A. Houck, N. Sipes, et al. “Zebrafish developmental screening of the ToxCast™ Phase I chemical library.Reproductive Toxicology (Elmsford, N.Y.) 33, no. 2 (April 2012): 174–87. https://doi.org/10.1016/j.reprotox.2011.10.018.
Padilla S, Corum D, Padnos B, Hunter DL, Beam A, Houck KA, et al. Zebrafish developmental screening of the ToxCast™ Phase I chemical library. Reproductive toxicology (Elmsford, NY). 2012 Apr;33(2):174–87.
Padilla, S., et al. “Zebrafish developmental screening of the ToxCast™ Phase I chemical library.Reproductive Toxicology (Elmsford, N.Y.), vol. 33, no. 2, Apr. 2012, pp. 174–87. Epmc, doi:10.1016/j.reprotox.2011.10.018.
Padilla S, Corum D, Padnos B, Hunter DL, Beam A, Houck KA, Sipes N, Kleinstreuer N, Knudsen T, Dix DJ, Reif DM. Zebrafish developmental screening of the ToxCast™ Phase I chemical library. Reproductive toxicology (Elmsford, NY). 2012 Apr;33(2):174–187.
Journal cover image

Published In

Reproductive toxicology (Elmsford, N.Y.)

DOI

EISSN

1873-1708

ISSN

0890-6238

Publication Date

April 2012

Volume

33

Issue

2

Start / End Page

174 / 187

Related Subject Headings

  • Zebrafish
  • Toxicology
  • Toxicity Tests
  • Teratogens
  • Small Molecule Libraries
  • Pesticides
  • Models, Animal
  • Environmental Pollutants
  • Embryo, Nonmammalian
  • Animals