Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis.

Journal Article (Journal Article)

Lysosomes are membrane-bound organelles that serve as the endpoint for endocytosis, phagocytosis, and autophagy, degrading the molecules, pathogens, and organelles localized within them. These cellular functions require intracellular transport. We use fluorescence microscopy to characterize the motion of lysosomes as a function of intracellular region, perinuclear or periphery, and lysosome diameter. Single-particle tracking data are complemented by changepoint identification and analysis of a mathematical model for state switching. We first classify lysosomal motion as motile or stationary. We then study how lysosome location and diameter affects the proportion of time spent in each state and quantify the speed during motile periods. We find that the proportion of time spent stationary is strongly region dependent, with significantly decreased motility in the perinuclear region. Increased lysosome diameter only slightly decreases speed. Overall, these results demonstrate the importance of decomposing particle trajectories into qualitatively different behaviors before conducting population-wide statistical analysis. Our results suggest that intracellular region is an important factor to consider in studies of intracellular transport.

Full Text

Duke Authors

Cited Authors

  • Rayens, NT; Cook, KJ; McKinley, SA; Payne, CK

Published Date

  • April 2022

Published In

Volume / Issue

  • 121 / 7

Start / End Page

  • 1205 - 1218

PubMed ID

  • 35202608

Pubmed Central ID

  • PMC9034247

Electronic International Standard Serial Number (EISSN)

  • 1542-0086

International Standard Serial Number (ISSN)

  • 0006-3495

Digital Object Identifier (DOI)

  • 10.1016/j.bpj.2022.02.032

Language

  • eng