A high-quality, long-read genome assembly of the endangered ring-tailed lemur (Lemur catta).

Journal Article (Journal Article)

BACKGROUND: The ring-tailed lemur (Lemur catta) is a charismatic strepsirrhine primate endemic to Madagascar. These lemurs are of particular interest, given their status as a flagship species and widespread publicity in the popular media. Unfortunately, a recent population decline has resulted in the census population decreasing to <2,500 individuals in the wild, and the species's classification as an endangered species by the IUCN. As is the case for most strepsirrhine primates, only a limited amount of genomic research has been conducted on L. catta, in part owing to the lack of genomic resources. RESULTS: We generated a new high-quality reference genome assembly for L. catta (mLemCat1) that conforms to the standards of the Vertebrate Genomes Project. This new long-read assembly is composed of Pacific Biosciences continuous long reads (CLR data), Optical Mapping Bionano reads, Arima HiC data, and 10X linked reads. The contiguity and completeness of the assembly are extremely high, with scaffold and contig N50 values of 90.982 and 10.570 Mb, respectively. Additionally, when compared to other high-quality primate assemblies, L. catta has the lowest reported number of Alu elements, which results predominantly from a lack of AluS and AluY elements. CONCLUSIONS: mLemCat1 is an excellent genomic resource not only for the ring-tailed lemur community, but also for other members of the Lemuridae family, and is the first very long read assembly for a strepsirrhine.

Full Text

Duke Authors

Cited Authors

  • Palmada-Flores, M; Orkin, JD; Haase, B; Mountcastle, J; Bertelsen, MF; Fedrigo, O; Kuderna, LFK; Jarvis, ED; Marques-Bonet, T

Published Date

  • April 1, 2022

Published In

Volume / Issue

  • 11 /

PubMed ID

  • 35365833

Pubmed Central ID

  • PMC8975718

Electronic International Standard Serial Number (EISSN)

  • 2047-217X

Digital Object Identifier (DOI)

  • 10.1093/gigascience/giac026

Language

  • eng

Conference Location

  • United States