Skip to main content

Hypoxia-induced blood-brain barrier dysfunction is prevented by pericyte-conditioned media via attenuated actomyosin contractility and claudin-5 stabilization.

Publication ,  Journal Article
Jamieson, JJ; Lin, Y; Malloy, N; Soto, D; Searson, PC; Gerecht, S
Published in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
May 2022

The blood-brain barrier (BBB) regulates molecular and cellular entry from the cerebrovasculature into the surrounding brain parenchyma. Many diseases of the brain are associated with dysfunction of the BBB, where hypoxia is a common stressor. However, the contribution of hypoxia to BBB dysfunction is challenging to study due to the complexity of the brain microenvironment. In this study, we used a BBB model with brain microvascular endothelial cells and pericytes differentiated from iPSCs to investigate the effect of hypoxia on barrier function. We found that hypoxia-induced barrier dysfunction is dependent upon increased actomyosin contractility and is associated with increased fibronectin fibrillogenesis. We propose a role for actomyosin contractility in mediating hypoxia-induced barrier dysfunction through modulation of junctional claudin-5. Our findings suggest pericytes may protect brain microvascular endothelial cells from hypoxic stresses and that pericyte-derived factors could be candidates for treatment of pathological barrier-forming tissues.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

DOI

EISSN

1530-6860

ISSN

0892-6638

Publication Date

May 2022

Volume

36

Issue

5

Start / End Page

e22331

Related Subject Headings

  • Pericytes
  • Humans
  • Endothelial Cells
  • Culture Media, Conditioned
  • Claudin-5
  • Cell Hypoxia
  • Blood-Brain Barrier
  • Biochemistry & Molecular Biology
  • Actomyosin
  • 3208 Medical physiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jamieson, J. J., Lin, Y., Malloy, N., Soto, D., Searson, P. C., & Gerecht, S. (2022). Hypoxia-induced blood-brain barrier dysfunction is prevented by pericyte-conditioned media via attenuated actomyosin contractility and claudin-5 stabilization. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 36(5), e22331. https://doi.org/10.1096/fj.202200010rr
Jamieson, John J., YingYu Lin, Nicholas Malloy, Daniel Soto, Peter C. Searson, and Sharon Gerecht. “Hypoxia-induced blood-brain barrier dysfunction is prevented by pericyte-conditioned media via attenuated actomyosin contractility and claudin-5 stabilization.FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology 36, no. 5 (May 2022): e22331. https://doi.org/10.1096/fj.202200010rr.
Jamieson JJ, Lin Y, Malloy N, Soto D, Searson PC, Gerecht S. Hypoxia-induced blood-brain barrier dysfunction is prevented by pericyte-conditioned media via attenuated actomyosin contractility and claudin-5 stabilization. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2022 May;36(5):e22331.
Jamieson, John J., et al. “Hypoxia-induced blood-brain barrier dysfunction is prevented by pericyte-conditioned media via attenuated actomyosin contractility and claudin-5 stabilization.FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol. 36, no. 5, May 2022, p. e22331. Epmc, doi:10.1096/fj.202200010rr.
Jamieson JJ, Lin Y, Malloy N, Soto D, Searson PC, Gerecht S. Hypoxia-induced blood-brain barrier dysfunction is prevented by pericyte-conditioned media via attenuated actomyosin contractility and claudin-5 stabilization. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2022 May;36(5):e22331.

Published In

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

DOI

EISSN

1530-6860

ISSN

0892-6638

Publication Date

May 2022

Volume

36

Issue

5

Start / End Page

e22331

Related Subject Headings

  • Pericytes
  • Humans
  • Endothelial Cells
  • Culture Media, Conditioned
  • Claudin-5
  • Cell Hypoxia
  • Blood-Brain Barrier
  • Biochemistry & Molecular Biology
  • Actomyosin
  • 3208 Medical physiology