Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus .

Journal Article (Journal Article)

Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus , one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination (HR) based on the CRISPR-Cas9 technology. This strategy opens a wide range of possibilities for the genetic modification of R. microsporus and will be useful for the study of mucormycosis. For complete details on the use and execution of this protocol, please refer to Lax et al. (2021).

Full Text

Duke Authors

Cited Authors

  • Lax, C; Navarro-Mendoza, MI; Pérez-Arques, C; Navarro, E; Nicolás, FE; Garre, V

Published Date

  • March 2022

Published In

Volume / Issue

  • 3 / 1

Start / End Page

  • 101237 -

PubMed ID

  • 35308131

Pubmed Central ID

  • PMC8927829

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

International Standard Serial Number (ISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2022.101237

Language

  • eng