Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus.
Publication
, Journal Article
Lax, C; Navarro-Mendoza, MI; Pérez-Arques, C; Navarro, E; Nicolás, FE; Garre, V
Published in: STAR protocols
March 2022
Here, we describe a reliable approach for targeted DNA integrations in the genome of R. microsporus, one of the main causal agents of mucormycosis. We provide a strategy for stable, targeted integration of DNA templates by homologous recombination (HR) based on the CRISPR-Cas9 technology. This strategy opens a wide range of possibilities for the genetic modification of R. microsporus and will be useful for the study of mucormycosis. For complete details on the use and execution of this protocol, please refer to Lax et al. (2021).
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Published In
STAR protocols
DOI
EISSN
2666-1667
ISSN
2666-1667
Publication Date
March 2022
Volume
3
Issue
1
Start / End Page
101237
Related Subject Headings
- Rhizopus
- Mucormycosis
- Homologous Recombination
- DNA
- CRISPR-Cas Systems
Citation
APA
Chicago
ICMJE
MLA
NLM
Lax, C., Navarro-Mendoza, M. I., Pérez-Arques, C., Navarro, E., Nicolás, F. E., & Garre, V. (2022). Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus. STAR Protocols, 3(1), 101237. https://doi.org/10.1016/j.xpro.2022.101237
Lax, Carlos, María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Eusebio Navarro, Francisco Esteban Nicolás, and Victoriano Garre. “Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus.” STAR Protocols 3, no. 1 (March 2022): 101237. https://doi.org/10.1016/j.xpro.2022.101237.
Lax C, Navarro-Mendoza MI, Pérez-Arques C, Navarro E, Nicolás FE, Garre V. Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus. STAR protocols. 2022 Mar;3(1):101237.
Lax, Carlos, et al. “Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus.” STAR Protocols, vol. 3, no. 1, Mar. 2022, p. 101237. Epmc, doi:10.1016/j.xpro.2022.101237.
Lax C, Navarro-Mendoza MI, Pérez-Arques C, Navarro E, Nicolás FE, Garre V. Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus. STAR protocols. 2022 Mar;3(1):101237.
Published In
STAR protocols
DOI
EISSN
2666-1667
ISSN
2666-1667
Publication Date
March 2022
Volume
3
Issue
1
Start / End Page
101237
Related Subject Headings
- Rhizopus
- Mucormycosis
- Homologous Recombination
- DNA
- CRISPR-Cas Systems