Pten-null tumors cohabiting the same lung display differential AKT activation and sensitivity to dietary restriction.

Journal Article (Journal Article)

PTEN loss is considered a biomarker for activated phosphoinositide 3-kinase (PI3K)/AKT, a pathway frequently mutated in cancer, and was recently shown to confer resistance to dietary restriction. Here, we show that Pten loss is not sufficient to drive AKT activation and resistance to dietary restriction in tumors with low growth factor receptor levels. We describe a murine Pten-null Kras-driven lung cancer model that harbors both dietary restriction-resistant, higher-grade, bronchiolar tumors with high AKT activity, and dietary restriction-sensitive, lower-grade, alveolar tumors with low AKT activity. We find that this phenotype is cell autonomous and that normal bronchiolar cells express higher levels of insulin-like growth factor-I receptor (IGF-IR) and of ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), an endoplasmic reticulum enzyme known to modulate growth factor receptor levels. Suppression of ENTPD5 is sufficient to decrease IGF-IR levels and sensitize bronchiolar tumor cells to serum in vitro and to dietary restriction in vivo. Furthermore, we find that a significant percentage of human non-small cell lung carcinomas (NSCLC) have low AKT activity despite PTEN loss.

Full Text

Duke Authors

Cited Authors

  • Curry, NL; Mino-Kenudson, M; Oliver, TG; Yilmaz, OH; Yilmaz, VO; Moon, JY; Jacks, T; Sabatini, DM; Kalaany, NY

Published Date

  • August 2013

Published In

Volume / Issue

  • 3 / 8

Start / End Page

  • 908 - 921

PubMed ID

  • 23719831

Pubmed Central ID

  • PMC3743121

Electronic International Standard Serial Number (EISSN)

  • 2159-8290

Digital Object Identifier (DOI)

  • 10.1158/2159-8290.CD-12-0507


  • eng

Conference Location

  • United States