CRISPR-Cas9-Guided Genome Engineering in C. elegans.

Journal Article (Journal Article)

The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system is successfully being used for efficient and targeted genome editing in various organisms, including the nematode C. elegans. Recent studies have developed various CRISPR-Cas9 approaches to enhance genome engineering via two major DNA double-strand break repair pathways: non-homologous end joining and homologous recombination. Here we describe a protocol for Cas9-mediated C. elegans genome editing together with single guide RNA (sgRNA) and repair template cloning, as well as injection methods required for delivering Cas9, sgRNAs, and repair template DNA into the C. elegans germline. © 2016 by John Wiley & Sons, Inc.

Full Text

Duke Authors

Cited Authors

  • Kim, H-M; Colaiácovo, MP

Published Date

  • July 2016

Published In

Volume / Issue

  • 115 /

Start / End Page

  • 31.7.1 - 31.7.18

PubMed ID

  • 27366893

Pubmed Central ID

  • PMC4932911

Electronic International Standard Serial Number (EISSN)

  • 1934-3647

International Standard Serial Number (ISSN)

  • 1934-3639

Digital Object Identifier (DOI)

  • 10.1002/cpmb.7

Language

  • eng