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CRISPR-Cas9-Guided Genome Engineering in C. elegans.

Publication ,  Journal Article
Kim, H-M; Colaiácovo, MP
Published in: Current protocols in molecular biology
July 2016

The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system is successfully being used for efficient and targeted genome editing in various organisms, including the nematode C. elegans. Recent studies have developed various CRISPR-Cas9 approaches to enhance genome engineering via two major DNA double-strand break repair pathways: non-homologous end joining and homologous recombination. Here we describe a protocol for Cas9-mediated C. elegans genome editing together with single guide RNA (sgRNA) and repair template cloning, as well as injection methods required for delivering Cas9, sgRNAs, and repair template DNA into the C. elegans germline. © 2016 by John Wiley & Sons, Inc.

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Published In

Current protocols in molecular biology

DOI

EISSN

1934-3647

ISSN

1934-3639

Publication Date

July 2016

Volume

115

Start / End Page

31.7.1 / 31.7.18

Related Subject Headings

  • RNA, Guide, CRISPR-Cas Systems
  • Homologous Recombination
  • Genome
  • Gene Editing
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Caenorhabditis elegans
  • CRISPR-Cas Systems
  • Biochemistry & Molecular Biology
  • Animals, Genetically Modified
  • Animals
 

Citation

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Kim, H.-M., & Colaiácovo, M. P. (2016). CRISPR-Cas9-Guided Genome Engineering in C. elegans. Current Protocols in Molecular Biology, 115, 31.7.1-31.7.18. https://doi.org/10.1002/cpmb.7
Kim, Hyun-Min, and Monica P. Colaiácovo. “CRISPR-Cas9-Guided Genome Engineering in C. elegans.Current Protocols in Molecular Biology 115 (July 2016): 31.7.1-31.7.18. https://doi.org/10.1002/cpmb.7.
Kim H-M, Colaiácovo MP. CRISPR-Cas9-Guided Genome Engineering in C. elegans. Current protocols in molecular biology. 2016 Jul;115:31.7.1-31.7.18.
Kim, Hyun-Min, and Monica P. Colaiácovo. “CRISPR-Cas9-Guided Genome Engineering in C. elegans.Current Protocols in Molecular Biology, vol. 115, July 2016, pp. 31.7.1-31.7.18. Epmc, doi:10.1002/cpmb.7.
Kim H-M, Colaiácovo MP. CRISPR-Cas9-Guided Genome Engineering in C. elegans. Current protocols in molecular biology. 2016 Jul;115:31.7.1-31.7.18.

Published In

Current protocols in molecular biology

DOI

EISSN

1934-3647

ISSN

1934-3639

Publication Date

July 2016

Volume

115

Start / End Page

31.7.1 / 31.7.18

Related Subject Headings

  • RNA, Guide, CRISPR-Cas Systems
  • Homologous Recombination
  • Genome
  • Gene Editing
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Caenorhabditis elegans
  • CRISPR-Cas Systems
  • Biochemistry & Molecular Biology
  • Animals, Genetically Modified
  • Animals