The DEK nuclear autoantigen is a secreted chemotactic factor.

Journal Article (Journal Article)

The nuclear DNA-binding protein DEK is an autoantigen that has been implicated in the regulation of transcription, chromatin architecture, and mRNA processing. We demonstrate here that DEK is actively secreted by macrophages and is also found in synovial fluid samples from patients with juvenile arthritis. Secretion of DEK is modulated by casein kinase 2, stimulated by interleukin-8, and inhibited by dexamethasone and cyclosporine A, consistent with a role as a proinflammatory molecule. DEK is secreted in both a free form and in exosomes, vesicular structures in which transcription-modulating factors such as DEK have not previously been found. Furthermore, DEK functions as a chemotactic factor, attracting neutrophils, CD8+ T lymphocytes, and natural killer cells. Therefore, the DEK autoantigen, previously described as a strictly nuclear protein, is secreted and can act as an extracellular chemoattractant, suggesting a direct role for DEK in inflammation.

Full Text

Duke Authors

Cited Authors

  • Mor-Vaknin, N; Punturieri, A; Sitwala, K; Faulkner, N; Legendre, M; Khodadoust, MS; Kappes, F; Ruth, JH; Koch, A; Glass, D; Petruzzelli, L; Adams, BS; Markovitz, DM

Published Date

  • December 2006

Published In

Volume / Issue

  • 26 / 24

Start / End Page

  • 9484 - 9496

PubMed ID

  • 17030615

Pubmed Central ID

  • PMC1698538

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.01030-06

Language

  • eng