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Functional domains of the ubiquitous chromatin protein DEK.

Publication ,  Journal Article
Kappes, F; Scholten, I; Richter, N; Gruss, C; Waldmann, T
Published in: Molecular and cellular biology
July 2004

DEK was originally described as a proto-oncogene protein and is now known to be a major component of metazoan chromatin. DEK is able to modify the structure of DNA by introducing supercoils. In order to find interaction partners and functional domains of DEK, we performed yeast two-hybrid screens and mutational analyses. Two-hybrid screening yielded C-terminal fragments of DEK, suggesting that DEK is able to multimerize. We could localize the domain to amino acids 270 to 350 and show that multimerization is dependent on phosphorylation by CK2 kinase in vitro. We also found two DNA binding domains of DEK, one on a fragment including amino acids 87 to 187 and containing the SAF-box DNA binding motif, which is located between amino acids 149 and 187. This region is sufficient to introduce supercoils into DNA. The second DNA binding domain is located between amino acids 270 and 350 and thus overlaps the multimerization domain. We show that the two DNA-interacting domains differ in their binding properties and in their abilities to respond to CK2 phosphorylation.

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Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

July 2004

Volume

24

Issue

13

Start / End Page

6000 / 6010

Related Subject Headings

  • Two-Hybrid System Techniques
  • Proto-Oncogene Mas
  • Protein Structure, Tertiary
  • Protein Serine-Threonine Kinases
  • Poly-ADP-Ribose Binding Proteins
  • Phosphorylation
  • Oncogene Proteins
  • Humans
  • Hela Cells
  • HeLa Cells
 

Citation

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Kappes, F., Scholten, I., Richter, N., Gruss, C., & Waldmann, T. (2004). Functional domains of the ubiquitous chromatin protein DEK. Molecular and Cellular Biology, 24(13), 6000–6010. https://doi.org/10.1128/mcb.24.13.6000-6010.2004
Kappes, Ferdinand, Ingo Scholten, Nicole Richter, Claudia Gruss, and Tanja Waldmann. “Functional domains of the ubiquitous chromatin protein DEK.Molecular and Cellular Biology 24, no. 13 (July 2004): 6000–6010. https://doi.org/10.1128/mcb.24.13.6000-6010.2004.
Kappes F, Scholten I, Richter N, Gruss C, Waldmann T. Functional domains of the ubiquitous chromatin protein DEK. Molecular and cellular biology. 2004 Jul;24(13):6000–10.
Kappes, Ferdinand, et al. “Functional domains of the ubiquitous chromatin protein DEK.Molecular and Cellular Biology, vol. 24, no. 13, July 2004, pp. 6000–10. Epmc, doi:10.1128/mcb.24.13.6000-6010.2004.
Kappes F, Scholten I, Richter N, Gruss C, Waldmann T. Functional domains of the ubiquitous chromatin protein DEK. Molecular and cellular biology. 2004 Jul;24(13):6000–6010.

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

July 2004

Volume

24

Issue

13

Start / End Page

6000 / 6010

Related Subject Headings

  • Two-Hybrid System Techniques
  • Proto-Oncogene Mas
  • Protein Structure, Tertiary
  • Protein Serine-Threonine Kinases
  • Poly-ADP-Ribose Binding Proteins
  • Phosphorylation
  • Oncogene Proteins
  • Humans
  • Hela Cells
  • HeLa Cells