Phosphorylation by protein kinase CK2 changes the DNA binding properties of the human chromatin protein DEK.
Publication
, Journal Article
Kappes, F; Damoc, C; Knippers, R; Przybylski, M; Pinna, LA; Gruss, C
Published in: Molecular and cellular biology
July 2004
We have examined the posttranslational modification of the human chromatin protein DEK and found that DEK is phosphorylated by the protein kinase CK2 in vitro and in vivo. Phosphorylation sites were mapped by quadrupole ion trap mass spectrometry and found to be clustered in the C-terminal region of the DEK protein. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase. Filter binding assays, as well as Southwestern analysis, demonstrate that phosphorylation weakens the binding of DEK to DNA. In vivo, however, phosphorylated DEK remains on chromatin. We present evidence that phosphorylated DEK is tethered to chromatin throughout the cell cycle by the un- or underphosphorylated form of DEK.
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Published In
Molecular and cellular biology
DOI
EISSN
1098-5549
ISSN
0270-7306
Publication Date
July 2004
Volume
24
Issue
13
Start / End Page
6011 / 6020
Related Subject Headings
- Resting Phase, Cell Cycle
- Protein Serine-Threonine Kinases
- Protein Processing, Post-Translational
- Poly-ADP-Ribose Binding Proteins
- Phosphorylation
- Oncogene Proteins
- Mass Spectrometry
- Humans
- Hela Cells
- HeLa Cells
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Kappes, F., Damoc, C., Knippers, R., Przybylski, M., Pinna, L. A., & Gruss, C. (2004). Phosphorylation by protein kinase CK2 changes the DNA binding properties of the human chromatin protein DEK. Molecular and Cellular Biology, 24(13), 6011–6020. https://doi.org/10.1128/mcb.24.13.6011-6020.2004
Kappes, Ferdinand, Catalina Damoc, Rolf Knippers, Michael Przybylski, Lorenzo A. Pinna, and Claudia Gruss. “Phosphorylation by protein kinase CK2 changes the DNA binding properties of the human chromatin protein DEK.” Molecular and Cellular Biology 24, no. 13 (July 2004): 6011–20. https://doi.org/10.1128/mcb.24.13.6011-6020.2004.
Kappes F, Damoc C, Knippers R, Przybylski M, Pinna LA, Gruss C. Phosphorylation by protein kinase CK2 changes the DNA binding properties of the human chromatin protein DEK. Molecular and cellular biology. 2004 Jul;24(13):6011–20.
Kappes, Ferdinand, et al. “Phosphorylation by protein kinase CK2 changes the DNA binding properties of the human chromatin protein DEK.” Molecular and Cellular Biology, vol. 24, no. 13, July 2004, pp. 6011–20. Epmc, doi:10.1128/mcb.24.13.6011-6020.2004.
Kappes F, Damoc C, Knippers R, Przybylski M, Pinna LA, Gruss C. Phosphorylation by protein kinase CK2 changes the DNA binding properties of the human chromatin protein DEK. Molecular and cellular biology. 2004 Jul;24(13):6011–6020.
Published In
Molecular and cellular biology
DOI
EISSN
1098-5549
ISSN
0270-7306
Publication Date
July 2004
Volume
24
Issue
13
Start / End Page
6011 / 6020
Related Subject Headings
- Resting Phase, Cell Cycle
- Protein Serine-Threonine Kinases
- Protein Processing, Post-Translational
- Poly-ADP-Ribose Binding Proteins
- Phosphorylation
- Oncogene Proteins
- Mass Spectrometry
- Humans
- Hela Cells
- HeLa Cells