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Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli.

Publication ,  Journal Article
Zhu, KW; Burton, SD; Nagai, MH; Silverman, JD; de March, CA; Wachowiak, M; Matsunami, H
Published in: Nat Commun
September 1, 2022

Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli in the mouse. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position.

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Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

September 1, 2022

Volume

13

Issue

1

Start / End Page

5137

Location

England

Related Subject Headings

  • Transcriptome
  • Smell
  • Receptors, Odorant
  • Olfactory Receptor Neurons
  • Olfactory Bulb
  • Mice
  • Axons
  • Animals
 

Citation

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Zhu, K. W., Burton, S. D., Nagai, M. H., Silverman, J. D., de March, C. A., Wachowiak, M., & Matsunami, H. (2022). Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli. Nat Commun, 13(1), 5137. https://doi.org/10.1038/s41467-022-32267-3
Zhu, Kevin W., Shawn D. Burton, Maira H. Nagai, Justin D. Silverman, Claire A. de March, Matt Wachowiak, and Hiroaki Matsunami. “Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli.Nat Commun 13, no. 1 (September 1, 2022): 5137. https://doi.org/10.1038/s41467-022-32267-3.
Zhu KW, Burton SD, Nagai MH, Silverman JD, de March CA, Wachowiak M, et al. Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli. Nat Commun. 2022 Sep 1;13(1):5137.
Zhu, Kevin W., et al. “Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli.Nat Commun, vol. 13, no. 1, Sept. 2022, p. 5137. Pubmed, doi:10.1038/s41467-022-32267-3.
Zhu KW, Burton SD, Nagai MH, Silverman JD, de March CA, Wachowiak M, Matsunami H. Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli. Nat Commun. 2022 Sep 1;13(1):5137.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

September 1, 2022

Volume

13

Issue

1

Start / End Page

5137

Location

England

Related Subject Headings

  • Transcriptome
  • Smell
  • Receptors, Odorant
  • Olfactory Receptor Neurons
  • Olfactory Bulb
  • Mice
  • Axons
  • Animals