Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli.

Journal Article (Journal Article)

Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli in the mouse. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position.

Full Text

Duke Authors

Cited Authors

  • Zhu, KW; Burton, SD; Nagai, MH; Silverman, JD; de March, CA; Wachowiak, M; Matsunami, H

Published Date

  • September 1, 2022

Published In

Volume / Issue

  • 13 / 1

Start / End Page

  • 5137 -

PubMed ID

  • 36050313

Pubmed Central ID

  • PMC9437035

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-022-32267-3


  • eng

Conference Location

  • England