Skip to main content
Journal cover image

Histone methyltransferase SETDB1 is required for prostate cancer cell proliferation, migration and invasion.

Publication ,  Journal Article
Sun, Y; Wei, M; Ren, S-C; Chen, R; Xu, W-D; Wang, F-B; Lu, J; Shen, J; Yu, Y-W; Hou, J-G; Xu, C-L; Huang, J-T; Sun, Y-H
Published in: Asian J Androl
2014

SETDB1 has been established as an oncogene in a number of human carcinomas. The present study was to evaluate the expression of SETDB1 in prostate cancer (PCa) tissues and cells and to preliminarily investigate the role of SETDB1 in prostate tumorigenesis in vitro. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were used to detect the expression of SETDB1 in PCa tissues, adjacent normal tissues, benign prostatic hyperplasia (BPH) tissues, PCa cell lines and normal prostate epithelial cells. The results suggested that SETDB1 was upregulated in human PCa tissues compared with normal tissues at the mRNA and protein levels. The role of SETDB1 in proliferation was analyzed with cell counting kit-8, colony-forming efficiency and flow cytometry assays. The results indicated that downregulation of SETDB1 by siRNA inhibited PCa cell growth, and induced G0/G1 cell cycle arrest. The PCa cell migration and invasion decreased by silcencing SETDB1 which were assessed by using in vitro scratch and transwell invasion assay respectively. Our data suggested that SETDB1 is overexpressed in human PCa. Silencing SETDB1 inhibited PCa cell proliferation, migration and invasion.

Duke Scholars

Published In

Asian J Androl

DOI

EISSN

1745-7262

Publication Date

2014

Volume

16

Issue

2

Start / End Page

319 / 324

Location

China

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Protein Methyltransferases
  • Prostatic Neoplasms
  • Obstetrics & Reproductive Medicine
  • Neoplasm Metastasis
  • Neoplasm Invasiveness
  • Male
  • Humans
  • Histone-Lysine N-Methyltransferase
  • Gene Silencing
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sun, Y., Wei, M., Ren, S.-C., Chen, R., Xu, W.-D., Wang, F.-B., … Sun, Y.-H. (2014). Histone methyltransferase SETDB1 is required for prostate cancer cell proliferation, migration and invasion. Asian J Androl, 16(2), 319–324. https://doi.org/10.4103/1008-682X.122812
Sun, Yi, Min Wei, Shan-Cheng Ren, Rui Chen, Wei-Dong Xu, Fu-Bo Wang, Ji Lu, et al. “Histone methyltransferase SETDB1 is required for prostate cancer cell proliferation, migration and invasion.Asian J Androl 16, no. 2 (2014): 319–24. https://doi.org/10.4103/1008-682X.122812.
Sun Y, Wei M, Ren S-C, Chen R, Xu W-D, Wang F-B, et al. Histone methyltransferase SETDB1 is required for prostate cancer cell proliferation, migration and invasion. Asian J Androl. 2014;16(2):319–24.
Sun, Yi, et al. “Histone methyltransferase SETDB1 is required for prostate cancer cell proliferation, migration and invasion.Asian J Androl, vol. 16, no. 2, 2014, pp. 319–24. Pubmed, doi:10.4103/1008-682X.122812.
Sun Y, Wei M, Ren S-C, Chen R, Xu W-D, Wang F-B, Lu J, Shen J, Yu Y-W, Hou J-G, Xu C-L, Huang J-T, Sun Y-H. Histone methyltransferase SETDB1 is required for prostate cancer cell proliferation, migration and invasion. Asian J Androl. 2014;16(2):319–324.
Journal cover image

Published In

Asian J Androl

DOI

EISSN

1745-7262

Publication Date

2014

Volume

16

Issue

2

Start / End Page

319 / 324

Location

China

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Protein Methyltransferases
  • Prostatic Neoplasms
  • Obstetrics & Reproductive Medicine
  • Neoplasm Metastasis
  • Neoplasm Invasiveness
  • Male
  • Humans
  • Histone-Lysine N-Methyltransferase
  • Gene Silencing