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Phosphatidylcholine transfer protein promotes apolipoprotein A-I-mediated lipid efflux in Chinese hamster ovary cells.

Publication ,  Journal Article
Baez, JM; Barbour, SE; Cohen, DE
Published in: J Biol Chem
February 22, 2002

Phosphatidylcholine transfer protein (PC-TP) is a cytosolic protein of unknown function that catalyzes intermembrane transfer of phosphatidylcholines in vitro. Using stably transfected CHO cells, we explored the influence of PC-TP on apolipoprotein A-I- and high density lipoprotein 3 (HDL(3))-mediated lipid efflux. In proportion to its cellular level of expression, PC-TP accelerated apolipoprotein A-I-mediated phospholipid and cholesterol efflux as pre-beta-HDL particles. PC-TP increased rates of efflux of both lipids by >2-fold but did not affect mRNA levels or the activity of ATP-binding cassette A1, a plasma membrane protein that regulates apolipoprotein A-I-mediated lipid efflux. Overexpression of PC-TP was associated with only slight increases in HDL(3)-mediated phospholipid efflux and no changes in cholesterol efflux. In scavenger receptor BI-overexpressing cells, PC-TP expression minimally influenced apolipoprotein A-I- or HDL(3)-mediated lipid efflux. PC-TP did not affect cellular phospholipid compositions, phosphatidylcholine contents, or phosphatidylcholine synthetic rates. These findings suggest that a physiological function of PC-TP is to replenish the plasma membrane with phosphatidylcholines that are removed during pre-beta-HDL particle formation due to the activity of ATP-binding cassette A1.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

February 22, 2002

Volume

277

Issue

8

Start / End Page

6198 / 6206

Location

United States

Related Subject Headings

  • Transfection
  • Recombinant Proteins
  • Phospholipid Transfer Proteins
  • Phosphatidylethanolamine Binding Protein
  • Phosphatidylcholines
  • Kinetics
  • Humans
  • Cytosol
  • Cricetinae
  • Carrier Proteins
 

Citation

APA
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ICMJE
MLA
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Baez, J. M., Barbour, S. E., & Cohen, D. E. (2002). Phosphatidylcholine transfer protein promotes apolipoprotein A-I-mediated lipid efflux in Chinese hamster ovary cells. J Biol Chem, 277(8), 6198–6206. https://doi.org/10.1074/jbc.M106799200
Baez, Juan M., Suzanne E. Barbour, and David E. Cohen. “Phosphatidylcholine transfer protein promotes apolipoprotein A-I-mediated lipid efflux in Chinese hamster ovary cells.J Biol Chem 277, no. 8 (February 22, 2002): 6198–6206. https://doi.org/10.1074/jbc.M106799200.
Baez, Juan M., et al. “Phosphatidylcholine transfer protein promotes apolipoprotein A-I-mediated lipid efflux in Chinese hamster ovary cells.J Biol Chem, vol. 277, no. 8, Feb. 2002, pp. 6198–206. Pubmed, doi:10.1074/jbc.M106799200.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

February 22, 2002

Volume

277

Issue

8

Start / End Page

6198 / 6206

Location

United States

Related Subject Headings

  • Transfection
  • Recombinant Proteins
  • Phospholipid Transfer Proteins
  • Phosphatidylethanolamine Binding Protein
  • Phosphatidylcholines
  • Kinetics
  • Humans
  • Cytosol
  • Cricetinae
  • Carrier Proteins