Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.

Journal Article (Journal Article)

Quantitative real-time PCR and next-generation sequencing (NGS) are invaluable techniques to analyze T cell receptor (Tcr) gene rearrangements in mouse lymphocyte populations. Although these approaches are powerful, they also have limitations that must be accounted for in experimental design and data interpretation. Here, we provide relevant background required for understanding these limitations and then outline established quantitative real-time PCR and NGS methods that can be used for analysis of mouse Tcra and Tcrb gene rearrangements in mice.

Full Text

Duke Authors

Cited Authors

  • Dauphars, DJ; Wu, G; Bassing, CH; Krangel, MS

Published Date

  • 2023

Published In

Volume / Issue

  • 2580 /

Start / End Page

  • 261 - 282

PubMed ID

  • 36374463

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-2740-2_16


  • eng

Conference Location

  • United States