Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.
Publication
, Journal Article
Dauphars, DJ; Wu, G; Bassing, CH; Krangel, MS
Published in: Methods Mol Biol
2023
Quantitative real-time PCR and next-generation sequencing (NGS) are invaluable techniques to analyze T cell receptor (Tcr) gene rearrangements in mouse lymphocyte populations. Although these approaches are powerful, they also have limitations that must be accounted for in experimental design and data interpretation. Here, we provide relevant background required for understanding these limitations and then outline established quantitative real-time PCR and NGS methods that can be used for analysis of mouse Tcra and Tcrb gene rearrangements in mice.
Duke Scholars
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2023
Volume
2580
Start / End Page
261 / 282
Location
United States
Related Subject Headings
- Receptors, Antigen, T-Cell, alpha-beta
- Polymerase Chain Reaction
- Mice
- Gene Rearrangement
- Developmental Biology
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Dauphars, D. J., Wu, G., Bassing, C. H., & Krangel, M. S. (2023). Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements. Methods Mol Biol, 2580, 261–282. https://doi.org/10.1007/978-1-0716-2740-2_16
Dauphars, Danielle J., Glendon Wu, Craig H. Bassing, and Michael S. Krangel. “Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.” Methods Mol Biol 2580 (2023): 261–82. https://doi.org/10.1007/978-1-0716-2740-2_16.
Dauphars DJ, Wu G, Bassing CH, Krangel MS. Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements. Methods Mol Biol. 2023;2580:261–82.
Dauphars, Danielle J., et al. “Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.” Methods Mol Biol, vol. 2580, 2023, pp. 261–82. Pubmed, doi:10.1007/978-1-0716-2740-2_16.
Dauphars DJ, Wu G, Bassing CH, Krangel MS. Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements. Methods Mol Biol. 2023;2580:261–282.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2023
Volume
2580
Start / End Page
261 / 282
Location
United States
Related Subject Headings
- Receptors, Antigen, T-Cell, alpha-beta
- Polymerase Chain Reaction
- Mice
- Gene Rearrangement
- Developmental Biology
- Animals
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences