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Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.

Publication ,  Journal Article
Dauphars, DJ; Wu, G; Bassing, CH; Krangel, MS
Published in: Methods Mol Biol
2023

Quantitative real-time PCR and next-generation sequencing (NGS) are invaluable techniques to analyze T cell receptor (Tcr) gene rearrangements in mouse lymphocyte populations. Although these approaches are powerful, they also have limitations that must be accounted for in experimental design and data interpretation. Here, we provide relevant background required for understanding these limitations and then outline established quantitative real-time PCR and NGS methods that can be used for analysis of mouse Tcra and Tcrb gene rearrangements in mice.

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Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2023

Volume

2580

Start / End Page

261 / 282

Location

United States

Related Subject Headings

  • Receptors, Antigen, T-Cell, alpha-beta
  • Polymerase Chain Reaction
  • Mice
  • Gene Rearrangement
  • Developmental Biology
  • Animals
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences
 

Citation

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Chicago
ICMJE
MLA
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Dauphars, D. J., Wu, G., Bassing, C. H., & Krangel, M. S. (2023). Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements. Methods Mol Biol, 2580, 261–282. https://doi.org/10.1007/978-1-0716-2740-2_16
Dauphars, Danielle J., Glendon Wu, Craig H. Bassing, and Michael S. Krangel. “Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.Methods Mol Biol 2580 (2023): 261–82. https://doi.org/10.1007/978-1-0716-2740-2_16.
Dauphars DJ, Wu G, Bassing CH, Krangel MS. Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements. Methods Mol Biol. 2023;2580:261–82.
Dauphars, Danielle J., et al. “Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements.Methods Mol Biol, vol. 2580, 2023, pp. 261–82. Pubmed, doi:10.1007/978-1-0716-2740-2_16.
Dauphars DJ, Wu G, Bassing CH, Krangel MS. Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements. Methods Mol Biol. 2023;2580:261–282.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2023

Volume

2580

Start / End Page

261 / 282

Location

United States

Related Subject Headings

  • Receptors, Antigen, T-Cell, alpha-beta
  • Polymerase Chain Reaction
  • Mice
  • Gene Rearrangement
  • Developmental Biology
  • Animals
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences