Cooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors.

Journal Article (Journal Article)

We investigated the role of chemokines in regulating T cell accumulation in solid tumors. CCL5 and CXCL9 overexpression was associated with CD8+ T cell infiltration in solid tumors. T cell infiltration required tumor cell-derived CCL5 and was amplified by IFN-γ-inducible, myeloid cell-secreted CXCL9. CCL5 and CXCL9 coexpression revealed immunoreactive tumors with prolonged survival and response to checkpoint blockade. Loss of CCL5 expression in human tumors was associated with epigenetic silencing through DNA methylation. Reduction of CCL5 expression caused tumor-infiltrating lymphocyte (TIL) desertification, whereas forced CCL5 expression prevented Cxcl9 expression and TILs loss, and attenuated tumor growth in mice through IFN-γ. The cooperation between tumor-derived CCL5 and IFN-γ-inducible CXCR3 ligands secreted by myeloid cells is key for orchestrating T cell infiltration in immunoreactive and immunoresponsive tumors.

Full Text

Duke Authors

Cited Authors

  • Dangaj, D; Bruand, M; Grimm, AJ; Ronet, C; Barras, D; Duttagupta, PA; Lanitis, E; Duraiswamy, J; Tanyi, JL; Benencia, F; Conejo-Garcia, J; Ramay, HR; Montone, KT; Powell, DJ; Gimotty, PA; Facciabene, A; Jackson, DG; Weber, JS; Rodig, SJ; Hodi, SF; Kandalaft, LE; Irving, M; Zhang, L; Foukas, P; Rusakiewicz, S; Delorenzi, M; Coukos, G

Published Date

  • June 10, 2019

Published In

Volume / Issue

  • 35 / 6

Start / End Page

  • 885 - 900.e10

PubMed ID

  • 31185212

Pubmed Central ID

  • PMC6961655

Electronic International Standard Serial Number (EISSN)

  • 1878-3686

Digital Object Identifier (DOI)

  • 10.1016/j.ccell.2019.05.004


  • eng

Conference Location

  • United States