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Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination.

Publication ,  Journal Article
Weidenhammer, LB; Liu, HQ; Luo, L; Williams, NT; Deland, K; Kirsch, DG; Reitman, ZJ
Published in: STAR Protoc
March 17, 2023

Genetically engineered mice are commonly used to model brainstem gliomas in pre-clinical research. One technique for inducing primary tumors in these genetically engineered mice involves delivering viral vectors containing the code for gene-editing proteins. We present a protocol for generating primary brainstem gliomas using the RCAS-TVA retroviral delivery system and the Cre/loxP gene editing system. We describe steps for transfecting and harvesting chicken fibroblast cells, intracranially injecting cells into mice, imaging primary tumors, and treating primary tumors with focal, image-guided brain irradiation. For complete details on the use and execution of this protocol, please refer to Deland et al. (2021).1.

Duke Scholars

Published In

STAR Protoc

DOI

EISSN

2666-1667

Publication Date

March 17, 2023

Volume

4

Issue

1

Start / End Page

102094

Location

United States

Related Subject Headings

  • Retroviridae
  • Recombination, Genetic
  • Mice
  • Glioma
  • Brain Stem Neoplasms
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Weidenhammer, L. B., Liu, H. Q., Luo, L., Williams, N. T., Deland, K., Kirsch, D. G., & Reitman, Z. J. (2023). Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination. STAR Protoc, 4(1), 102094. https://doi.org/10.1016/j.xpro.2023.102094
Weidenhammer, Loren B., Harrison Q. Liu, Lixia Luo, Nerissa T. Williams, Katherine Deland, David G. Kirsch, and Zachary J. Reitman. “Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination.STAR Protoc 4, no. 1 (March 17, 2023): 102094. https://doi.org/10.1016/j.xpro.2023.102094.
Weidenhammer LB, Liu HQ, Luo L, Williams NT, Deland K, Kirsch DG, et al. Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination. STAR Protoc. 2023 Mar 17;4(1):102094.
Weidenhammer, Loren B., et al. “Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination.STAR Protoc, vol. 4, no. 1, Mar. 2023, p. 102094. Pubmed, doi:10.1016/j.xpro.2023.102094.
Weidenhammer LB, Liu HQ, Luo L, Williams NT, Deland K, Kirsch DG, Reitman ZJ. Inducing primary brainstem gliomas in genetically engineered mice using RCAS/TVA retroviruses and Cre/loxP recombination. STAR Protoc. 2023 Mar 17;4(1):102094.
Journal cover image

Published In

STAR Protoc

DOI

EISSN

2666-1667

Publication Date

March 17, 2023

Volume

4

Issue

1

Start / End Page

102094

Location

United States

Related Subject Headings

  • Retroviridae
  • Recombination, Genetic
  • Mice
  • Glioma
  • Brain Stem Neoplasms
  • Animals