Skip to main content

Cloning and characterization of developmental endothelial locus-1: an embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor.

Publication ,  Journal Article
Hidai, C; Zupancic, T; Penta, K; Mikhail, A; Kawana, M; Quertermous, EE; Aoka, Y; Fukagawa, M; Matsui, Y; Platika, D; Auerbach, R; Hogan, BL ...
Published in: Genes Dev
January 1, 1998

We have taken advantage of an enhancer trap event in a line of transgenic mice to identify a unique developmentally regulated endothelial cell locus (Del1). The protein encoded in this locus contains three EGF-like repeats homologous to those in Notch and related proteins, including an EGF-like repeat that contains an RGD motif, and two discoidin I-like domains. Del1 is shown to be a matrix protein and to promote adhesion of endothelial cells through interaction with the alphavbeta3 integrin receptor. Embryonic endothelial-like yolk sac cells expressing recombinant Del1 protein, or grown on an extracellular matrix containing Del1 protein, are inhibited from forming vascular-like structures. Expression of Del1 protein in the chick chorioallantoic membrane leads to loss of vascular integrity and promotes vessel remodeling. Del1 is thus a new ligand for the alphavbeta3 integrin receptor and may function to regulate vascular morphogenesis or remodeling in embryonic development.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

January 1, 1998

Volume

12

Issue

1

Start / End Page

21 / 33

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Sequence Homology, Amino Acid
  • Receptors, Vitronectin
  • RNA
  • Protein Binding
  • Neovascularization, Physiologic
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Intercellular Signaling Peptides and Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hidai, C., Zupancic, T., Penta, K., Mikhail, A., Kawana, M., Quertermous, E. E., … Quertermous, T. (1998). Cloning and characterization of developmental endothelial locus-1: an embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor. Genes Dev, 12(1), 21–33. https://doi.org/10.1101/gad.12.1.21
Hidai, C., T. Zupancic, K. Penta, A. Mikhail, M. Kawana, E. E. Quertermous, Y. Aoka, et al. “Cloning and characterization of developmental endothelial locus-1: an embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor.Genes Dev 12, no. 1 (January 1, 1998): 21–33. https://doi.org/10.1101/gad.12.1.21.
Hidai C, Zupancic T, Penta K, Mikhail A, Kawana M, Quertermous EE, et al. Cloning and characterization of developmental endothelial locus-1: an embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor. Genes Dev. 1998 Jan 1;12(1):21–33.
Hidai, C., et al. “Cloning and characterization of developmental endothelial locus-1: an embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor.Genes Dev, vol. 12, no. 1, Jan. 1998, pp. 21–33. Pubmed, doi:10.1101/gad.12.1.21.
Hidai C, Zupancic T, Penta K, Mikhail A, Kawana M, Quertermous EE, Aoka Y, Fukagawa M, Matsui Y, Platika D, Auerbach R, Hogan BL, Snodgrass R, Quertermous T. Cloning and characterization of developmental endothelial locus-1: an embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor. Genes Dev. 1998 Jan 1;12(1):21–33.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

January 1, 1998

Volume

12

Issue

1

Start / End Page

21 / 33

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Sequence Homology, Amino Acid
  • Receptors, Vitronectin
  • RNA
  • Protein Binding
  • Neovascularization, Physiologic
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Intercellular Signaling Peptides and Proteins