ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae.
Publication
, Journal Article
Ramirez, JA; Vacata, V; McCusker, JH; Haber, JE; Mortimer, RK; Owen, WG; Lecar, H
Published in: Proc Natl Acad Sci U S A
October 1989
A mutant in the plasma membrane H+-ATPase gene of the yeast Saccharomyces cerevisiae with a reduced H+-ATPase activity, when examined at the single-channel level with the patch-clamp technique, was found to exhibit K+ channels activated by intracellular application of ATP. In the parent strain, the same channel, identified by its conductance and selectivity, is not activated by ATP. This activity in the mutant is blocked by the ATPase inhibitor N,N'-dicyclohexylcarbodiimide. ADP and the ATP analog adenosine 5'-[gamma-[35S]thio]triphosphate do not activate the channel. These findings suggest a tight physical coupling between the plasma membrane ATPase and the K+ channel.
Duke Scholars
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
October 1989
Volume
86
Issue
20
Start / End Page
7866 / 7870
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae
- Proton-Translocating ATPases
- Potassium Channels
- Mutation
- Models, Theoretical
- Mathematics
- Kinetics
- Electric Conductivity
- Dicyclohexylcarbodiimide
- Adenosine Triphosphate
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Ramirez, J. A., Vacata, V., McCusker, J. H., Haber, J. E., Mortimer, R. K., Owen, W. G., & Lecar, H. (1989). ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A, 86(20), 7866–7870. https://doi.org/10.1073/pnas.86.20.7866
Ramirez, J. A., V. Vacata, J. H. McCusker, J. E. Haber, R. K. Mortimer, W. G. Owen, and H. Lecar. “ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae.” Proc Natl Acad Sci U S A 86, no. 20 (October 1989): 7866–70. https://doi.org/10.1073/pnas.86.20.7866.
Ramirez JA, Vacata V, McCusker JH, Haber JE, Mortimer RK, Owen WG, et al. ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7866–70.
Ramirez, J. A., et al. “ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae.” Proc Natl Acad Sci U S A, vol. 86, no. 20, Oct. 1989, pp. 7866–70. Pubmed, doi:10.1073/pnas.86.20.7866.
Ramirez JA, Vacata V, McCusker JH, Haber JE, Mortimer RK, Owen WG, Lecar H. ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7866–7870.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
October 1989
Volume
86
Issue
20
Start / End Page
7866 / 7870
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae
- Proton-Translocating ATPases
- Potassium Channels
- Mutation
- Models, Theoretical
- Mathematics
- Kinetics
- Electric Conductivity
- Dicyclohexylcarbodiimide
- Adenosine Triphosphate