ATP-sensitive K+ channels in a plasma membrane H+-ATPase mutant of the yeast Saccharomyces cerevisiae.
Journal Article (Journal Article)
A mutant in the plasma membrane H+-ATPase gene of the yeast Saccharomyces cerevisiae with a reduced H+-ATPase activity, when examined at the single-channel level with the patch-clamp technique, was found to exhibit K+ channels activated by intracellular application of ATP. In the parent strain, the same channel, identified by its conductance and selectivity, is not activated by ATP. This activity in the mutant is blocked by the ATPase inhibitor N,N'-dicyclohexylcarbodiimide. ADP and the ATP analog adenosine 5'-[gamma-[35S]thio]triphosphate do not activate the channel. These findings suggest a tight physical coupling between the plasma membrane ATPase and the K+ channel.
Full Text
Duke Authors
Cited Authors
- Ramirez, JA; Vacata, V; McCusker, JH; Haber, JE; Mortimer, RK; Owen, WG; Lecar, H
Published Date
- October 1989
Published In
Volume / Issue
- 86 / 20
Start / End Page
- 7866 - 7870
PubMed ID
- 2530577
Pubmed Central ID
- PMC298172
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.86.20.7866
Language
- eng
Conference Location
- United States