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Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation.

Publication ,  Journal Article
Feng, S; Shen, Y; Sullivan, JA; Rubio, V; Xiong, Y; Sun, T-P; Deng, XW
Published in: The Plant cell
July 2004

Ubiquitin/proteasome-mediated protein degradation controls various developmental pathways in eukaryotes. Cullin-containing complexes are both versatile and abundant groups of RING family ubiquitin E3 ligases, whose activities are subject to control by RUB/Nedd8 (for related to ubiquitin/neural precursor cell-expressed developmentally downregulated 8) modification of their cullin subunits. Here, we report the identification of an Arabidopsis thaliana counterpart of human CAND1 (cullin-associated and neddylation-dissociated) and demonstrate that it can preferentially interact with unmodified CUL1. The Arabidopsis cand1-1 null mutant displays distinct phenotypes, including late flowering, aerial rosettes, floral organ defects, low fertility, dwarfism, loss of apical dominance, and altered responses to multiple plant hormones. Molecular analyses show that many of these defects are because of compromised activity of CUL1-containing ubiquitin E3 ligases, indicating that CAND1 is required for their optimal activity. Furthermore, the cand1-1 mutant displays a partial constitutive photomorphogenic phenotype and has defects in HY5 degradation in the absence of light, a process mediated by a different RING family E3, COP1. Thus, our data provides genetic support for a critical role of CAND1 in regulating various ubiquitin E3 ligases and their targeted cellular and developmental pathways.

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Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

July 2004

Volume

16

Issue

7

Start / End Page

1870 / 1882

Related Subject Headings

  • Ubiquitin-Protein Ligases
  • Ubiquitin
  • Plants, Genetically Modified
  • Plant Biology & Botany
  • Phylogeny
  • Phenotype
  • Mutation
  • Mutagenesis, Insertional
  • Molecular Sequence Data
  • Flowers
 

Citation

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Feng, S., Shen, Y., Sullivan, J. A., Rubio, V., Xiong, Y., Sun, T.-P., & Deng, X. W. (2004). Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation. The Plant Cell, 16(7), 1870–1882. https://doi.org/10.1105/tpc.021949
Feng, Suhua, Yunping Shen, James A. Sullivan, Vicente Rubio, Yue Xiong, Tai-ping Sun, and Xing Wang Deng. “Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation.The Plant Cell 16, no. 7 (July 2004): 1870–82. https://doi.org/10.1105/tpc.021949.
Feng, Suhua, et al. “Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation.The Plant Cell, vol. 16, no. 7, July 2004, pp. 1870–82. Epmc, doi:10.1105/tpc.021949.

Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

July 2004

Volume

16

Issue

7

Start / End Page

1870 / 1882

Related Subject Headings

  • Ubiquitin-Protein Ligases
  • Ubiquitin
  • Plants, Genetically Modified
  • Plant Biology & Botany
  • Phylogeny
  • Phenotype
  • Mutation
  • Mutagenesis, Insertional
  • Molecular Sequence Data
  • Flowers