Multiple gene genealogies reveal recent dispersion and hybridization in the human pathogenic fungus Cryptococcus neoformans.

Journal Article (Journal Article)

Cryptococcus neoformans (= Filobasidiella neoformans) is a significant emerging fungal pathogen of humans. To understand the evolution of this pathogen, 34 strains were obtained from various locations around the world and fragments of four genes were sequenced from each. These strains represented all three varieties and five serotypes. The four sequenced genes are: (i) the mitochondrial large ribosomal subunit RNA; (ii) the internal transcribed spacer region of the nuclear rRNA, including ITS1, 5.8S rRNA subunit and ITS2; (iii) orotidine monophosphate pyrophosphorylase; and (iv) diphenol oxidase. Phylogenetic analyses indicated considerable divergence among lineages, which corresponded to the current classification of C. neoformans into three varieties. However, there is no apparent phylogeographic pattern. Significant incongruences were observed among gene genealogies. The analyses indicated that the major lineages in C. neoformans diverged tens of millions of years ago but have undergone recent dispersion and hybridization.

Full Text

Duke Authors

Cited Authors

  • Xu, J; Vilgalys, R; Mitchell, TG

Published Date

  • October 2000

Published In

Volume / Issue

  • 9 / 10

Start / End Page

  • 1471 - 1481

PubMed ID

  • 11050543

International Standard Serial Number (ISSN)

  • 0962-1083

Digital Object Identifier (DOI)

  • 10.1046/j.1365-294x.2000.01021.x


  • eng

Conference Location

  • England