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Insulin-like growth factor binding protein-3 induces early apoptosis in malignant prostate cancer cells and inhibits tumor formation in vivo.

Publication ,  Journal Article
Devi, GR; Sprenger, CC; Plymate, SR; Rosenfeld, RG
Published in: Prostate
May 1, 2002

BACKGROUND: Insulin-like growth factor binding protein (IGFBP-3) levels are significantly reduced in malignant prostate epithelial cells. In this study, we evaluated the role of endogenous IGFBP-3 on prostate cancer cell growth and tumorigenesis. METHODS: IGFBP-3 was re-expressed by stable transfection of human IGFBP-3 cDNA in a model of human prostate cancer, M12, a malignant subline in which IGFBP-3 levels are undetectable in comparison to the parent epithelial cell, P69. Effect of IGFBP-3 re-expression (M12-BP-3) on growth kinetics, morphology, propensity to apoptosis, and in vivo tumor formation were studied. RESULTS: M12-BP-3 cells secreted IGFBP-3 and growth arrested at a cell density that was threefold lower than control cells and this was associated with marked alteration in cell morphology. Control cells when grown in conditioned media secreted by M12-BP-3 also showed altered morphology compared to when cultured in IGFBP-3-immunodepleted conditioned media. The M12-BP-3 clones showed altered mitochondrial membrane potential, increased PARP cleavage, increase in sub-G1 peak, decreased levels of neuron specific enolase, and decreased tumor formation in athymic, nude mice. CONCLUSIONS: These data suggest that IGFBP-3 induces early apoptosis and has potential tumor suppressive effect in prostate cancer. Prostate 51: 141-152, 2002.

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Published In

Prostate

DOI

ISSN

0270-4137

Publication Date

May 1, 2002

Volume

51

Issue

2

Start / End Page

141 / 152

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • Male
  • Insulin-Like Growth Factor Binding Protein 3
  • Immunohistochemistry
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Epithelial Cells
  • Electrophoresis, Polyacrylamide Gel
 

Citation

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Devi, G. R., Sprenger, C. C., Plymate, S. R., & Rosenfeld, R. G. (2002). Insulin-like growth factor binding protein-3 induces early apoptosis in malignant prostate cancer cells and inhibits tumor formation in vivo. Prostate, 51(2), 141–152. https://doi.org/10.1002/pros.10068
Devi, Gayathri R., Cynthia C. Sprenger, Stephen R. Plymate, and Ron G. Rosenfeld. “Insulin-like growth factor binding protein-3 induces early apoptosis in malignant prostate cancer cells and inhibits tumor formation in vivo.Prostate 51, no. 2 (May 1, 2002): 141–52. https://doi.org/10.1002/pros.10068.
Devi, Gayathri R., et al. “Insulin-like growth factor binding protein-3 induces early apoptosis in malignant prostate cancer cells and inhibits tumor formation in vivo.Prostate, vol. 51, no. 2, May 2002, pp. 141–52. Pubmed, doi:10.1002/pros.10068.
Journal cover image

Published In

Prostate

DOI

ISSN

0270-4137

Publication Date

May 1, 2002

Volume

51

Issue

2

Start / End Page

141 / 152

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • Male
  • Insulin-Like Growth Factor Binding Protein 3
  • Immunohistochemistry
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Epithelial Cells
  • Electrophoresis, Polyacrylamide Gel