Clinical isolates of measles virus use CD46 as a cellular receptor.

Published

Journal Article

Laboratory strains of measles viruses (MV), such as Edmonston and Halle, use the complement regulatory protein CD46 as a cell surface receptor. The receptor usage of clinical isolates of MV, however, remains unclear. Receptor usage by primary patient isolates of MV was compared to isolates that had been passaged on a variety of tissue culture cell lines. All of the isolates could infect cells in a CD46-dependent manner, but their tropism was restricted according to cell type (e.g., lymphocytes versus fibroblasts). The results indicate that patient isolates that have not been adapted to tissue culture cell lines use CD46 as a receptor. In addition, passaging primary MV patient isolates in B95-8 cells selected variants that had alternate receptor usage compared to the original isolate. Thus, changes in receptor usage by MV are dependent upon the cell type used for isolation. Furthermore, our results confirm the relevance of the CD46 receptor to natural measles infection.

Full Text

Duke Authors

Cited Authors

  • Manchester, M; Eto, DS; Valsamakis, A; Liton, PB; Fernandez-Muñoz, R; Rota, PA; Bellini, WJ; Forthal, DN; Oldstone, MB

Published Date

  • May 2000

Published In

Volume / Issue

  • 74 / 9

Start / End Page

  • 3967 - 3974

PubMed ID

  • 10756008

Pubmed Central ID

  • 10756008

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.74.9.3967-3974.2000

Language

  • eng