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In vivo catabolism of heparin cofactor II and its complex with thrombin: evidence for a common receptor-mediated clearance pathway for three serine proteinase inhibitors.

Publication ,  Journal Article
Pratt, CW; Church, FC; Pizzo, SV
Published in: Arch Biochem Biophys
April 1988

The plasma clearance of 125I-labeled human heparin cofactor II and its complex with thrombin was studied in mice to determine whether a specific mechanism exists for the catabolism of the inhibitor-proteinase complex. Initial studies demonstrated that murine plasma contains a heparin cofactor II-like inhibitor as shown by the presence of a dermatan sulfate-sensitive thrombin inhibitor. Human heparin cofactor II cleared from the circulation of mice with an apparent half-life of 80 min while heparin cofactor II-thrombin complexes cleared with an apparent half-life of only 10 min. The specificity of the clearance mechanism was investigated by clearance competition studies involving coinjection of excess unlabeled heparin cofactor II-alpha-thrombin, antithrombin III-alpha-thrombin, or alpha 1-proteinase inhibitor-elastase, and by tissue distribution studies. The results demonstrated that the clearance of 125I-labeled heparin cofactor II-alpha-thrombin is a receptor-mediated process, and that the same hepatocyte receptor system recognizes complexes containing heparin cofactor II, antithrombin III, and alpha 1-proteinase inhibitor.

Duke Scholars

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

April 1988

Volume

262

Issue

1

Start / End Page

111 / 117

Location

United States

Related Subject Headings

  • alpha 1-Antitrypsin
  • Tissue Distribution
  • Thrombin
  • Serine Proteinase Inhibitors
  • Protease Inhibitors
  • Pancreatic Elastase
  • Mice
  • Metabolic Clearance Rate
  • Macromolecular Substances
  • Leukocyte Elastase
 

Citation

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Pratt, C. W., Church, F. C., & Pizzo, S. V. (1988). In vivo catabolism of heparin cofactor II and its complex with thrombin: evidence for a common receptor-mediated clearance pathway for three serine proteinase inhibitors. Arch Biochem Biophys, 262(1), 111–117. https://doi.org/10.1016/0003-9861(88)90173-7
Pratt, C. W., F. C. Church, and S. V. Pizzo. “In vivo catabolism of heparin cofactor II and its complex with thrombin: evidence for a common receptor-mediated clearance pathway for three serine proteinase inhibitors.Arch Biochem Biophys 262, no. 1 (April 1988): 111–17. https://doi.org/10.1016/0003-9861(88)90173-7.
Pratt, C. W., et al. “In vivo catabolism of heparin cofactor II and its complex with thrombin: evidence for a common receptor-mediated clearance pathway for three serine proteinase inhibitors.Arch Biochem Biophys, vol. 262, no. 1, Apr. 1988, pp. 111–17. Pubmed, doi:10.1016/0003-9861(88)90173-7.
Journal cover image

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

April 1988

Volume

262

Issue

1

Start / End Page

111 / 117

Location

United States

Related Subject Headings

  • alpha 1-Antitrypsin
  • Tissue Distribution
  • Thrombin
  • Serine Proteinase Inhibitors
  • Protease Inhibitors
  • Pancreatic Elastase
  • Mice
  • Metabolic Clearance Rate
  • Macromolecular Substances
  • Leukocyte Elastase