Simple and sensitive assay employing stable reagents for quantification of plasminogen activator.

Journal Article (Journal Article)

A simple and sensitive indirect assay for quantifying plasminogen activator using [3H]-casein as substrate is described. The assay has been used to measure plasminogen activators from various sources including bacteria, cultured cells, and human plasma. The assay is linear with respect to concentration of plasminogen activator and time of incubation and is independent of concentration of plasminogen. The assay can routinely be used to quantify as few as 8 X 10(-3) international units ml-1 of streptokinase. Results obtained with the [3H]-casein assay correlate well with those obtained by the fibrin plate method (R = 0.83 by Spearman's ranked co-efficient of correlation). The reagents are extremely stable and easily stored. The ease with which the assay can be performed gives the clinical laboratory the potential to test large numbers of patients upon short notice and within a short period of time.

Full Text

Duke Authors

Cited Authors

  • Lewis, JG; Pizzo, SV; Adams, DO

Published Date

  • October 1, 1981

Published In

Volume / Issue

  • 76 / 4

Start / End Page

  • 403 - 409

PubMed ID

  • 7197461

International Standard Serial Number (ISSN)

  • 0002-9173

Digital Object Identifier (DOI)

  • 10.1093/ajcp/76.4.403


  • eng

Conference Location

  • England