The localisation of radiolabelled murine monoclonal antibody 81C6 and its Fab fragment in human glioma xenografts in athymic mice.

Published

Journal Article

The localisation of the radioiodinated Fab fragment of monoclonal antibody (Mab) 81C6, reactive with a glioma-associated extracellular matrix antigen, was studied in athymic mice bearing subcutaneous and intracranial xenografts of D-54 MG glioma cells. In vitro 81C6 Fab showed a marked loss of immunoreactivity and affinity for antigen compared to intact Mab 81C6. In vivo, the plasma half-life of 81C6 Fab was 7.0 hours compared to 2.1 days for 81C6. 81C6 Fab levels in tumours peaked at 2.6-3.8% injected dose/g in 2-6 h; Mab 81C6 reached 33.9% dose/g at 48 h. Localisation indices and tumour:tissue ratios were superior for Mab 81C6. Estimated radiation doses to tumour and normal tissues were lower for 131I-81C6 Fab than 131I-81C6. To realise the theoretical benefits of fragments as localising agents, Fab fragments of higher immunoreactivity and affinity, or bivalent F(ab')2 fragments are required.

Full Text

Duke Authors

Cited Authors

  • Colapinto, EV; Lee, YS; Humphrey, PA; Zalutsky, MR; Friedman, HS; Bullard, DE; Bigner, DD

Published Date

  • 1988

Published In

Volume / Issue

  • 2 / 2

Start / End Page

  • 179 - 191

PubMed ID

  • 3267302

Pubmed Central ID

  • 3267302

International Standard Serial Number (ISSN)

  • 0268-8697

Language

  • eng

Conference Location

  • England