Infrequent p53 gene mutations in medulloblastomas.


Journal Article

Cytogenetic and molecular studies of medulloblastomas have demonstrated frequent loss of sequences from the short arm of chromosome 17, possibly implicating loss or inactivation of the p53 tumor suppressor gene. We amplified exons 5 through 8 of the p53 gene by the polymerase chain reaction technique. These segments, which encompass the regions usually mutated in human tumors, were sequenced to search for p53 mutations in 12 medulloblastoma tumors, 8 xenografts, and 3 permanent cell lines. Mutation of the p53 gene was found in only 1 of 3 cell lines tested and in none of the xenografts or primary tumors studied. Our results suggest that p53 is mutated in an unusual way or that a second tumor suppressor gene on the short arm of chromosome 17 is involved in the pathogenesis of medulloblastoma.

Full Text

Duke Authors

Cited Authors

  • Saylors, RL; Sidransky, D; Friedman, HS; Bigner, SH; Bigner, DD; Vogelstein, B; Brodeur, GM

Published Date

  • September 1, 1991

Published In

Volume / Issue

  • 51 / 17

Start / End Page

  • 4721 - 4723

PubMed ID

  • 1873817

Pubmed Central ID

  • 1873817

International Standard Serial Number (ISSN)

  • 0008-5472


  • eng

Conference Location

  • United States