Infrequent p53 gene mutations in medulloblastomas.
Cytogenetic and molecular studies of medulloblastomas have demonstrated frequent loss of sequences from the short arm of chromosome 17, possibly implicating loss or inactivation of the p53 tumor suppressor gene. We amplified exons 5 through 8 of the p53 gene by the polymerase chain reaction technique. These segments, which encompass the regions usually mutated in human tumors, were sequenced to search for p53 mutations in 12 medulloblastoma tumors, 8 xenografts, and 3 permanent cell lines. Mutation of the p53 gene was found in only 1 of 3 cell lines tested and in none of the xenografts or primary tumors studied. Our results suggest that p53 is mutated in an unusual way or that a second tumor suppressor gene on the short arm of chromosome 17 is involved in the pathogenesis of medulloblastoma.
Duke Scholars
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Related Subject Headings
- Tumor Cells, Cultured
- Oncology & Carcinogenesis
- Mutation
- Medulloblastoma
- Humans
- Genes, p53
- Chromosomes, Human, Pair 17
- Cerebellar Neoplasms
- 3211 Oncology and carcinogenesis
- 3101 Biochemistry and cell biology
Citation
Published In
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tumor Cells, Cultured
- Oncology & Carcinogenesis
- Mutation
- Medulloblastoma
- Humans
- Genes, p53
- Chromosomes, Human, Pair 17
- Cerebellar Neoplasms
- 3211 Oncology and carcinogenesis
- 3101 Biochemistry and cell biology